Protein Info for Psyr_2273 in Pseudomonas syringae pv. syringae B728a

Annotation: L-glutamine synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 63% identical to GMAS_METMY: Glutamate--methylamine ligase from Methylovorus mays

KEGG orthology group: K01915, glutamine synthetase [EC: 6.3.1.2] (inferred from 100% identity to psb:Psyr_2273)

MetaCyc: 63% identical to glutamate--methylamine ligase monomer (Methylovorus mays)
Glutamate--methylamine ligase. [EC: 6.3.4.12]; Glutamate--ethylamine ligase. [EC: 6.3.4.12, 6.3.1.6]

Predicted SEED Role

"Glutamine synthetase type I (EC 6.3.1.2)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis or Glutamine synthetases or Peptidoglycan Biosynthesis (EC 6.3.1.2)

MetaCyc Pathways

L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
ammonia assimilation cycle III (3/3 steps found)
methylamine degradation II (3/3 steps found)
ammonia assimilation cycle I (2/2 steps found)
L-glutamine biosynthesis I (1/1 steps found)
L-glutamate and L-glutamine biosynthesis (5/7 steps found)
L-aspartate degradation II (aerobic) (2/3 steps found)
L-aspartate degradation III (anaerobic) (2/3 steps found)
superpathway of ammonia assimilation (plants) (2/3 steps found)
ammonia assimilation cycle II (1/2 steps found)
superpathway of trimethylamine degradation (3/6 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.1.2

Use Curated BLAST to search for 6.3.1.2 or 6.3.1.6 or 6.3.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZU56 at UniProt or InterPro

Protein Sequence (444 amino acids)

>Psyr_2273 L-glutamine synthetase (Pseudomonas syringae pv. syringae B728a)
MLPPETQRLIEQHGIKYVLAQFVDIHGSAKTKSVPVTGLEMVAEDGAGFAGFAICGMGME
PHGPDFMARGDLSSLTPVPWQPGYGRVVCIGHVDGKPWPYDSRYVLQQQVERLSQRGWSL
NTGLEPEFSLFKRDVTGSLQMVDASDNLDKPCYDYKGLSRSREFLERLTEALQPVGFDIY
QIDHEDANGQFEINYTYSEAMESADRFTFFRMAAGEIANDMGMICSFMPKPDPKRAGNGM
HFHLSIASASNKNLFHDASDPSGMGLSTLAYHFAAGLLAHGPALCAFAAPTVNSYKRLVV
GNSLSGATWAPAFIAFGANNRSAMVRVPYGRLEFRLPDAGCNPYLVSAAIIAAGLDGIDR
QLEIDHVCNENLYKLSLEEIAARGIKTLPQSLNEACDALQADPLFGEVLGSEIVDEFIRL
KRMEWVEYSRHVSDWEVKRYIEFF