Protein Info for Psyr_2197 in Pseudomonas syringae pv. syringae B728a

Annotation: urease, Metallo peptidase, MEROPS family M38

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 570 PF00449: Urease_alpha" amino acids 3 to 123 (121 residues), 157.6 bits, see alignment E=2.6e-50 TIGR01792: urease, alpha subunit" amino acids 4 to 569 (566 residues), 816.4 bits, see alignment E=7e-250 PF01979: Amidohydro_1" amino acids 130 to 456 (327 residues), 226 bits, see alignment E=1.3e-70 PF07969: Amidohydro_3" amino acids 234 to 457 (224 residues), 33.7 bits, see alignment E=4.5e-12

Best Hits

Swiss-Prot: 100% identical to URE11_PSEU2: Urease subunit alpha 1 (ureC1) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K01428, urease subunit alpha [EC: 3.5.1.5] (inferred from 100% identity to psb:Psyr_2197)

MetaCyc: 58% identical to urease alpha subunit (Sinorhizobium meliloti Rm2011)
Urease. [EC: 3.5.1.5]

Predicted SEED Role

"Urease alpha subunit (EC 3.5.1.5)" in subsystem Urea decomposition (EC 3.5.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.5

Use Curated BLAST to search for 3.5.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZUD2 at UniProt or InterPro

Protein Sequence (570 amino acids)

>Psyr_2197 urease, Metallo peptidase, MEROPS family M38 (Pseudomonas syringae pv. syringae B728a)
MATMTRKEYAAMYGPTTGDAVRLGDTSLLAEVEFDHSVPGDECLHGGGKTLRDGMGLMPG
HDSVDGALDMLICNALIIDPVIGIVKGDIGIKDGKIVAIGKAGNPQIMDGVHPQLICGVA
TTVRDAEGLIVTPGGIDVHVHFDSAQLCDHALAAGLTTLIGGSLGPITVGIDCGGEWNVG
KMLQAAEAWPINFGFLGRGNSSKPESLLGQLRGGCLGLKIHEDWGAMPAVIDTCLKVADE
YDFQVQLHTDTLNESGFLEDTLAAIGDRTIHMYHTEGAGGGHAPDIISVAGKSNCIPSST
NPTNPYTVNTFDEHLDMIMVCHHLNPDVPEDVAFAESRVRPQTIAAEDILHDTGAISILG
SDSQGMGRINEVICRTWQLASKMKDQRGRLPEETTALGDNERIKRYIAKYTINAARVFGI
DSYIGSLEPGKLADLVLWRPAFFGIKPELVVKGGFIVHAVMGDSAASLYTCEPLVMRPQW
GAFGEAKQALSVNFVNRLAVEADTATRLGLKKQLLPAFGTRTLRKSDMLHNDACPDIRVD
PQTFDVYADGERLHCEPVSEVPLAQRYMLR