Protein Info for Psyr_1781 in Pseudomonas syringae pv. syringae B728a
Annotation: Beta-glucosidase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01188, beta-glucosidase [EC: 3.2.1.21] (inferred from 100% identity to psb:Psyr_1781)Predicted SEED Role
"glycosyl hydrolase, family 3"
MetaCyc Pathways
- neolinustatin bioactivation (2/3 steps found)
- linamarin degradation (1/2 steps found)
- lotaustralin degradation (1/2 steps found)
- linustatin bioactivation (2/4 steps found)
- cellulose degradation II (fungi) (1/3 steps found)
- coumarin biosynthesis (via 2-coumarate) (2/5 steps found)
- α-tomatine degradation (1/6 steps found)
- firefly bioluminescence (2/14 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.2.1.21
Use Curated BLAST to search for 3.2.1.21
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q4ZVJ3 at UniProt or InterPro
Protein Sequence (906 amino acids)
>Psyr_1781 Beta-glucosidase (Pseudomonas syringae pv. syringae B728a) MNDVRNNSVSMIKQGLGLSLLALGIAQAGSVFAVTPMTGNEVEARVGAMLDNMTQSEKIN FSRVDDGRMIPKLDKFNLQGTVAYDSAMGVLVGGKTFGAQYPSPSALAATWSINRAKEFG LAIGYETRIAGGQQMLSPAINLYRTPFNGRAAEYMSGEDPFLGAVLAPAVTNGIQVQGVQ AAAKHYLMNEQEANRHYLDVKIDERAMQELYLPGFESLVKNSNIASIMCGYNKINGEYAC ENHHLITDILKGQWGFQGNVMSDFNSVQDAFKGAWAGTDIDMPSGLQFTEAKLLPYVWNG QLHQNVIDDKVRRNLRALVSYGFDKGINKAQPLEHREYGQLAALNVARESIVLLRNEPVD GQKPVLPLSRNARIAVIGDMAMQPPTAPFGTAYSAPASYVTEVSGLKQLASDPSNVDYLP QMSLNPKSSVWYQPGSDQQAISNTGVKAEYYANTSLSGDPVATRVEPGVNLDWITNTNVT DYGSSAVNGYSPSAGSFSARFTGKIKPTITGPQVFKVRADGAYKLWINDELVLEDEGAQV SFDLIPVVPRTVKTPNLKAGSEYNVRLEYRRLKGNFTPVLGGMNGVQMSWAALRPPKNLG DYDAVVVAAGNNFEYEGESLDRPFELPQFQSELIGFIQKANPKTIVVLHGGGGMDMQPWA DKVAASLHAWYPGQQGGQALAEILFGKVNPSGKLPITLDKKAQDNPSYASYSNPVPYYFS SPNPPVEMSYSEGLYLGYRGYDKKHTKPLYPFGFGLSYTTYAYSDLKLSSNVLAPGATIQ ASFKVTNTGDKAGFEVAQLYVQPSKPLVDRPEKELKGFSKVYLKPGESKTVTIALDSRSF AYYSANSVSWNVDPGKFKVLVGKDSENLTLDRTVVALYPEQLTTRDSNPLPIPLRKAVQV KAEQAY