Protein Info for Psyr_1672 in Pseudomonas syringae pv. syringae B728a
Annotation: L-threonine ammonia-lyase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 58% identical to ILVA_BURM1: L-threonine dehydratase biosynthetic IlvA (ilvA) from Burkholderia multivorans (strain ATCC 17616 / 249)
KEGG orthology group: K01754, threonine dehydratase [EC: 4.3.1.19] (inferred from 100% identity to psb:Psyr_1672)MetaCyc: 54% identical to threonine deaminase (Escherichia coli K-12 substr. MG1655)
Threonine ammonia-lyase. [EC: 4.3.1.19]
Predicted SEED Role
"Threonine dehydratase biosynthetic (EC 4.3.1.19)" in subsystem Branched-Chain Amino Acid Biosynthesis (EC 4.3.1.19)
MetaCyc Pathways
- superpathway of branched chain amino acid biosynthesis (17/17 steps found)
- superpathway of L-isoleucine biosynthesis I (13/13 steps found)
- L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
- L-methionine degradation II (2/3 steps found)
- L-threonine degradation I (4/6 steps found)
- L-threonine degradation V (1/2 steps found)
- superpathway of L-threonine metabolism (11/18 steps found)
- hypoglycin biosynthesis (4/14 steps found)
- cyclosporin A biosynthesis (2/15 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 4.3.1.19
Use Curated BLAST to search for 4.3.1.19
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q4ZVV2 at UniProt or InterPro
Protein Sequence (511 amino acids)
>Psyr_1672 L-threonine ammonia-lyase (Pseudomonas syringae pv. syringae B728a) MSQDPHALLNYYARKILTSRVYDVAIETPLQGARQLSERLGNQVLLKREDLQPVFSFKIR GAYNKLAQLPAEQTARGVVTASAGNHAQGLALAARELGIKATIVMPRTTPEIKVEGVRSR GATVVLHGDSFPEALAYSLKLVDEQGFVYIHPYDDPDTIAGQGTVAMEILRQQPGQLDAI FVPVGGGGLIAGIAAYVKYLRPEIKVIGVEPDDSNCLQAAMAAGERVVLSQVGLFADGVA VAQIGHHTFEVCRHYVDEVITVSTDEICAAIKDIYDDTRSITEPSGALGVAGIKKYVEQR GVSGQTLVAIDSGANVNFDRLRHVAERAELGEGREAIIAVTIPEQPGSFKAFCEAIGKRQ ITEFNYRYHTDREAHIFVGVQTHPENDPRSALIASLTGQGFPVLDLTDNELAKLHIRHMV GGHAERVDDEVVLRFEFPERPGALFNFLNRLGGRWTISMFHYRNHGAADGRVVAGLVVPE EERHLVGAALDEIGYRYWDESENPAYRLFLG