Protein Info for Psyr_1454 in Pseudomonas syringae pv. syringae B728a

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 719 transmembrane" amino acids 17 to 36 (20 residues), see Phobius details amino acids 42 to 60 (19 residues), see Phobius details TIGR03754: conjugative coupling factor TraD, PFGI-1 class" amino acids 7 to 654 (648 residues), 1082.3 bits, see alignment E=0 TIGR03743: conjugative coupling factor TraD, SXT/TOL subfamily" amino acids 7 to 654 (648 residues), 858.3 bits, see alignment E=3.5e-262 PF12696: TraG-D_C" amino acids 506 to 633 (128 residues), 47.9 bits, see alignment E=1.3e-16

Best Hits

KEGG orthology group: None (inferred from 100% identity to psb:Psyr_1454)

Predicted SEED Role

"Type IV secretory pathway, VirD4 components"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZWG8 at UniProt or InterPro

Protein Sequence (719 amino acids)

>Psyr_1454 hypothetical protein (Pseudomonas syringae pv. syringae B728a)
MAHDYAIESLLRPAVELYTVYVCAAGAFLCVFAPWAFALTPLFGIVTSAGFLALGLVRLK
QAWQVLRYRRNIRRLPHYTMTSKEVPVSNQRLFIGLGFRWQQRHTQRLMDTYLPKYASYV
EATSLFRAARRFEERAEFAPYPVRLLARATSWDVPINPVRPLPPVGGLPRLHGIEPYEEN
VSLPLGERVGHSIVLGTTRVGKTRLAELFITQDIRRKKHGKHEVVIVFDPKGDADLLKRM
YLEAKRAGRLNEFYVFHLGWPDHSARYNAVGRFGRISEVATRIAGQLSGEGNSAAFREFA
WRFVNIIARALVALGRRPDYLQIQQHVINIEGIFQEYASKYFDEYDPKAWEAIVAIEGKL
NEKNVPFNMKGRPFRVVAIDQYLSQTRVADPVMDGLRSAVRYDKTYFDKIVASLLPLLEK
LTTGRMAELISPDYQDVNDPRPIFDWMQVVRKKAVVYIGLDALSDTEVAAAVGNSMFSDL
VSVAGHIYKFGVDDGLPGGVPGTKVPINLHADEFNELMGEEFIPMINKGGGAGMQVTAYT
QTMSDIEAKIGNKAKAGQVIGNFNNVFMLRVRETSTAELLTNQLPKVQVFTSTPASSAND
SATGNTAFTSSSHDQVQQVSVPMIEPAHVVGLPKGQCFALMEGGNLWKIRMPLPGHDPDE
VMPADLQALAESMRQTYQVGKVDDTGWWSAPGYQTLNESLPADLLDDFKKIAVAPEDAA