Protein Info for Psyr_1392 in Pseudomonas syringae pv. syringae B728a

Annotation: SSU ribosomal protein S12P methylthiotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 447 TIGR00089: radical SAM methylthiotransferase, MiaB/RimO family" amino acids 11 to 444 (434 residues), 329.8 bits, see alignment E=2.4e-102 TIGR01125: ribosomal protein S12 methylthiotransferase RimO" amino acids 11 to 443 (433 residues), 574.8 bits, see alignment E=1.3e-176 PF00919: UPF0004" amino acids 11 to 86 (76 residues), 71.7 bits, see alignment E=6.4e-24 PF04055: Radical_SAM" amino acids 148 to 326 (179 residues), 83.4 bits, see alignment E=3.3e-27 PF18693: TRAM_2" amino acids 383 to 446 (64 residues), 69.7 bits, see alignment E=2.9e-23

Best Hits

Swiss-Prot: 100% identical to RIMO_PSEU2: Ribosomal protein S12 methylthiotransferase RimO (rimO) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K14441, ribosomal protein S12 methylthiotransferase [EC: 2.-.-.-] (inferred from 100% identity to psb:Psyr_1392)

MetaCyc: 70% identical to ribosomal protein S12 methylthiotransferase RimO (Escherichia coli K-12 substr. MG1655)
RXN0-6366 [EC: 2.8.4.4]

Predicted SEED Role

"Ribosomal protein S12p Asp88 (E. coli) methylthiotransferase" in subsystem Ribosomal protein S12p Asp methylthiotransferase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.-.-.-

Use Curated BLAST to search for 2.-.-.- or 2.8.4.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZWM9 at UniProt or InterPro

Protein Sequence (447 amino acids)

>Psyr_1392 SSU ribosomal protein S12P methylthiotransferase (Pseudomonas syringae pv. syringae B728a)
MSTVTTPSAPKVGFVSLGCPKALVDSERILTQLRMEGYEVVATYEDADVVVVNTCGFIDT
AKAESLEVIGEAIKENGKVIVTGCMGVDASVIRNVHPSVLSVTGPQQYEQVVNAVHDVVP
PRKDHNPLIDLVPPQGVKLTPRHYAYLKISEGCNHSCSFCIIPSMRGKLVSRPVGDVLDE
AKRLVKSGVKELLVISQDTSAYGVDVKYRTGFWDGQPVKTRMTELCQALGSMGVWVRLHY
VYPYPHVDELIPLMAAGKILPYLDIPFQHASPKILKLMKRPAFEDKTLARIKNWREQCPD
LIIRSTFIVGFPGETEEDFQYLLDWLTEAQLDRVGCFQYSPVEGAPANLLDAAIVPDDVK
QDRWDRFMAHQQAISAARLQMKIGKEIEVLIDEVDDRGAVGRCFFDAPEIDGNVFIGLDD
GSTVQPGDKIMCRVTDADEYDLWAEML