Protein Info for Psyr_1278 in Pseudomonas syringae pv. syringae B728a

Annotation: Citrate-proton symport

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 456 transmembrane" amino acids 58 to 75 (18 residues), see Phobius details amino acids 84 to 106 (23 residues), see Phobius details amino acids 118 to 138 (21 residues), see Phobius details amino acids 144 to 170 (27 residues), see Phobius details amino acids 182 to 207 (26 residues), see Phobius details amino acids 218 to 238 (21 residues), see Phobius details amino acids 272 to 295 (24 residues), see Phobius details amino acids 307 to 328 (22 residues), see Phobius details amino acids 337 to 356 (20 residues), see Phobius details amino acids 362 to 386 (25 residues), see Phobius details amino acids 398 to 418 (21 residues), see Phobius details amino acids 429 to 448 (20 residues), see Phobius details PF00083: Sugar_tr" amino acids 46 to 253 (208 residues), 94.5 bits, see alignment E=7.4e-31 amino acids 280 to 454 (175 residues), 42.8 bits, see alignment E=3.4e-15 PF07690: MFS_1" amino acids 49 to 316 (268 residues), 49.3 bits, see alignment E=3.5e-17 amino acids 307 to 449 (143 residues), 43.9 bits, see alignment E=1.6e-15 TIGR00883: MFS transporter, metabolite:H+ symporter (MHS) family protein" amino acids 51 to 441 (391 residues), 451.3 bits, see alignment E=1.7e-139

Best Hits

Swiss-Prot: 54% identical to YHJE_ECOLI: Inner membrane metabolite transport protein YhjE (yhjE) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to psb:Psyr_1278)

Predicted SEED Role

"Permeases of the major facilitator superfamily"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZWZ3 at UniProt or InterPro

Protein Sequence (456 amino acids)

>Psyr_1278 Citrate-proton symport (Pseudomonas syringae pv. syringae B728a)
MHACRNTVDADRRVDCQEIRMTSTPLSREDHDAHAKPVNSTTRVATASFIGTAIEFYDFY
VYATAAALVIGPVFFPQTSGTAQMLSSFLTFGIAFLARPLGSALFGHFGDRIGRKSTLVA
SLLLMGICTTLIGVLPGYATIGAWAPILLCVLRFGQGLGLGGEWGGAALLATENAPKGKR
AWFGMFPQLGPSIGFLAANGLFLALAMTLDDEQFRAWGWRIPFLLSAVLVMVGLYVRLKL
EETPVFAKAMARHQRVSLPIVETFSQHWKPMLLGAASMVVCYALFYISTVFSLSYGVATL
GYTRETFLGLLCFAVLFMAAATPLAALASDRFGRKPVLIAGGVLAILSGFTMEPLLTHGS
TWAVALFLCIELFLMGITFAPMGALLPELFPTQVRYTGASAAYNLGGIVGASVAPFFAQK
LVAMGGLSWVGGYVSAAALISVLAVLSLKETRDREL