Protein Info for Psyr_1128 in Pseudomonas syringae pv. syringae B728a

Annotation: Acriflavin resistance protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1034 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details transmembrane" amino acids 339 to 357 (19 residues), see Phobius details amino acids 364 to 385 (22 residues), see Phobius details amino acids 391 to 414 (24 residues), see Phobius details amino acids 435 to 455 (21 residues), see Phobius details amino acids 467 to 494 (28 residues), see Phobius details amino acids 528 to 547 (20 residues), see Phobius details amino acids 859 to 877 (19 residues), see Phobius details amino acids 884 to 904 (21 residues), see Phobius details amino acids 910 to 933 (24 residues), see Phobius details amino acids 960 to 980 (21 residues), see Phobius details amino acids 988 to 1012 (25 residues), see Phobius details PF00873: ACR_tran" amino acids 9 to 1013 (1005 residues), 990.3 bits, see alignment E=1.5e-301 PF03176: MMPL" amino acids 304 to 497 (194 residues), 47.9 bits, see alignment E=1.5e-16 PF02355: SecD_SecF_C" amino acids 340 to 493 (154 residues), 25 bits, see alignment E=1.8e-09

Best Hits

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 100% identity to psb:Psyr_1128)

Predicted SEED Role

"Cation/multidrug efflux pump"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZXE0 at UniProt or InterPro

Protein Sequence (1034 amino acids)

>Psyr_1128 Acriflavin resistance protein (Pseudomonas syringae pv. syringae B728a)
MNGRGSVSAWCIDHPVATLLLTFALVLLGVIAFPRLPIAPLPEAEFPTIQVTAQLPGASP
ETMASSVATPLEVQFSAIPGMTQMTSSSALGSTNLTLQFTLNKSIDTAAQEVQAAINTAA
GRLPADLPSLPTWRKVNPADSPVLILSVSSSLIPGTELSDVTETILARQLSQIEGVGQVF
ITGQQRPAIRVQAAPEKLAALGLTLADIRLAVQQTSLNLAKGALYGKDSISTLSSNDQLF
KPQDYAQLIVSYKNGAPVQLKDVARVVAGSENAYVKAWSGDQQGVNIAIFRQPGANIVDT
VDRIQRELPRLQEMLPASVDVSVLNDRTRTIRASLHEVEMTLMIAVLLVVAVMALFLRQL
SATLIVSAVLGVSLIASFAMMYLFGFSLNNLTLVAIVVAVGFVVDDAIVVVENIHRHLEA
GQDMREAAIKGSGEIGFTVVSISFSLVAAFIPLLFMGGVVGRLFKEFALTATATILISVV
VSLTLAPTLAALFMRAPTHHPHQKPGFGERLLASYERGLRKALAHQRLMLGVFGLTLALA
VVGYILIPKGFFPVQDTAFALGTTEAAADISYPDMVEKHLELAKIVGADPAVLAFSHSVG
VSGSNQTIANGRFWISLKPRAERDVSVSEFIDRLRPKLAKVPGIVLYLRAGQDINLSSGP
SRSQYQYVLKSNDGELLNTWTQRLTEKLRSNPAFRDMSNDLQLGGSVTHIDIDRSAAARF
GLTTADVDQALYDAFGQRQISEYQTEVNQYKVILELDAQQRGKAESLAYFYLRSPLTSEM
VPLSALAKVSAPRRGPLSISHDGMFPAANLSFNLAPGVALGDAVRMLDQAKNEIGMPASI
IGSFQGAAQAFQSSLANQPWLILAALVAVYIILGVLYESFVHPLTIISTLPSAGIGALLL
LWMMGQDFSIMALIGVVLLIGIVKKNGILLVDFALQAQREQGLTPQEAIYEACITRFRPI
IMTTLAALLGALPLMLGFGVGSELRQPLGIAVVGGLLVSQMLTLFTTPVIYLQLERLFHR
RHPAPDAPALALHK