Protein Info for Psyr_0994 in Pseudomonas syringae pv. syringae B728a

Annotation: Metal-dependent phosphohydrolase, HD subdomain protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 393 transmembrane" amino acids 148 to 165 (18 residues), see Phobius details PF11871: DUF3391" amino acids 2 to 117 (116 residues), 93.9 bits, see alignment E=2e-30 PF13487: HD_5" amino acids 145 to 300 (156 residues), 110.2 bits, see alignment E=1.4e-35 TIGR00277: HDIG domain" amino acids 147 to 240 (94 residues), 28.4 bits, see alignment E=6e-11 PF01966: HD" amino acids 149 to 271 (123 residues), 52.8 bits, see alignment E=7.1e-18

Best Hits

Swiss-Prot: 54% identical to CDPD1_PSEAE: Cyclic di-GMP phosphodiesterase PA4108 (PA4108) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 100% identity to psb:Psyr_0994)

Predicted SEED Role

"HDIG domain protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZXS2 at UniProt or InterPro

Protein Sequence (393 amino acids)

>Psyr_0994 Metal-dependent phosphohydrolase, HD subdomain protein (Pseudomonas syringae pv. syringae B728a)
MLKKIPVADLALGMYIHKICRRWTNDPFWIGLVEVMLDDVEVLERIQQSGTKEVWIETGK
SRVLAPRPSTPSASDDTTPASLEKEMLRARLICGRARDAVMCMFTEARMGRAMDVDNVQL
LVEEISNSILRHPHALISLSRLKTSDEYTYMHSVAVCALMVALAKRMGMPDDQVREAGVA
GLMHDVGKMMIAPAVLNKPGRLTRDEFEIMKAHPELGVKILMDTQPVAASVIDVCLHHHE
KVDGSGYPHGLKGDQISIFARMGAVCDVYDAITSDRPYKKGWGVAHSIREMASWQGHFDD
VVFQSFVKTVGIYPIGTLVRLESGRLGVVVEQGQASLLKPKVKVFMSARTGKTFAAQVID
LDCPSDPDRIARIEKTTDWGLEEVDTLWAGSPA