Protein Info for Psyr_0865 in Pseudomonas syringae pv. syringae B728a

Annotation: General substrate transporter:Major facilitator superfamily

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 402 transmembrane" amino acids 12 to 34 (23 residues), see Phobius details amino acids 49 to 71 (23 residues), see Phobius details amino acids 81 to 105 (25 residues), see Phobius details amino acids 111 to 130 (20 residues), see Phobius details amino acids 139 to 158 (20 residues), see Phobius details amino acids 170 to 192 (23 residues), see Phobius details amino acids 213 to 233 (21 residues), see Phobius details amino acids 248 to 269 (22 residues), see Phobius details amino acids 276 to 295 (20 residues), see Phobius details amino acids 301 to 322 (22 residues), see Phobius details amino acids 334 to 356 (23 residues), see Phobius details amino acids 363 to 383 (21 residues), see Phobius details PF07690: MFS_1" amino acids 19 to 339 (321 residues), 127.7 bits, see alignment E=2.6e-41

Best Hits

Swiss-Prot: 100% identical to SOTB_PSEU2: Probable sugar efflux transporter (sotB) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K03445, MFS transporter, DHA1 family, purine ribonucleoside efflux pump (inferred from 100% identity to psb:Psyr_0865)

MetaCyc: 60% identical to L-arabinose exporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-40

Predicted SEED Role

"Sugar efflux transporter SotB"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZY49 at UniProt or InterPro

Protein Sequence (402 amino acids)

>Psyr_0865 General substrate transporter:Major facilitator superfamily (Pseudomonas syringae pv. syringae B728a)
MITSPPNEQAGSWLGVFALALAAFIFNTTEFVPIGLLSNIGQSFEMTPAQVGLMLTIYAW
VVSLMSLPMMLATRNIERRKLLMFVFGLFVVSHVLSAIAPSFAILLVSRVGIAFAHAVFW
SVTASLAVRIAPPGKQVQALGLLATGTSLAMVLGIPLGRVLGEALGWRTTFLGIAAIAAL
VVFLLVRALPLLPSQNSGSLRSLPILFKRPRLMAIYLLTAIVVTAHFTAYSYIEPFTQTV
SLLSGEMTTILLLVFGGAGIMGSIIFSLFSDRFPNGLLITAIGTLTLCLLMLLPLSGDAV
TLGTLTIIWGMAIMCFGLTLQARVLSLAPDATDVAMALFSGIFNIGIGGGALLGSVVSSH
LGVANVGIVGGLLALGGLGLCCYTTHHFGKVRPAAEMQAESK