Protein Info for Psyr_0859 in Pseudomonas syringae pv. syringae B728a

Annotation: Carbonate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00484: Pro_CA
  • amino acids 38 to 188 (151 residues), 177.4 bits, E=1.3e-56

Best Hits

Swiss-Prot: 56% identical to CAN_ECOLI: Carbonic anhydrase 2 (can) from Escherichia coli (strain K12)

KEGG orthology group: K01673, carbonic anhydrase [EC: 4.2.1.1] (inferred from 99% identity to pst:PSPTO_0994)

MetaCyc: 56% identical to carbonic anhydrase 2 (Escherichia coli K-12 substr. MG1655)
Carbonate dehydratase. [EC: 4.2.1.1]

Predicted SEED Role

"Carbonic anhydrase (EC 4.2.1.1)" in subsystem Carboxysome or Cyanate hydrolysis (EC 4.2.1.1)

MetaCyc Pathways

• cyanate degradation (3/3 steps found)
• CO₂ fixation into oxaloacetate (anaplerotic) (2/2 steps found)
• C4 photosynthetic carbon assimilation cycle, NAD-ME type (8/11 steps found)
• C4 photosynthetic carbon assimilation cycle, NADP-ME type (4/7 steps found)
• C4 photosynthetic carbon assimilation cycle, PEPCK type (9/14 steps found)
• 3-hydroxypropanoate cycle (7/13 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (9/18 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (9/18 steps found)
• glyoxylate assimilation (5/13 steps found)
• superpathway of the 3-hydroxypropanoate cycle (7/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)

KEGG Metabolic Maps

• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.1

Use Curated BLAST to search for 4.2.1.1

Sequence Analysis Tools

See Q4ZY55 at UniProt or InterPro

Protein Sequence (212 amino acids)

>Psyr_0859 Carbonate dehydratase (Pseudomonas syringae pv. syringae B728a)
MNELQDLIDNNARWADAIKQEDPEFFAKLARQQTPEFLWIGCSDARVPANEIVGMLPGDL
FVHRNVANVVLHTDLNCLSVIQYAVDVLKVKHILVTGHYGCGGVRASMQDRQFGLIDGWL
RTIRDLYYENRELLAKLPTEEERVDRLCELNVIQQVANVGHTSIVQNAWHRGQSLSVHGC
IYGIKDGRWKSLDVTISGFEQLPPQYRLRPQD