Protein Info for Psyr_0733 in Pseudomonas syringae pv. syringae B728a

Annotation: Relaxase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 318 PF07514: TraI_2" amino acids 20 to 302 (283 residues), 405.2 bits, see alignment E=9e-126 TIGR03760: integrating conjugative element relaxase, PFGI-1 class" amino acids 20 to 240 (221 residues), 282.3 bits, see alignment E=1.3e-88

Best Hits

KEGG orthology group: None (inferred from 100% identity to psb:Psyr_0733)

Predicted SEED Role

"Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZYH5 at UniProt or InterPro

Protein Sequence (318 amino acids)

>Psyr_0733 Relaxase (Pseudomonas syringae pv. syringae B728a)
MLSFFTRRKASPISSNAAAGFFKPESPDALLSTSRRRQLIENIWQRTSLPREQFEALYMQ
AFKSYAALVQHLPASENHHHAYHGGMLDHGLEIVAYALKIRQMYLLPIGAPPESQAAQSE
AWSAASAYGALVHDLGKIAVDVKVELADGTIWHPWHGPLDQPYRFKYVKGRDYRLHGAAS
SLIYSHVIPAKALDWLSGFPELWSQLVFAFAFAFAGQYEHADILGEIVSQADQASVAQEL
GGNPGKAMSAPKQSIQRQLAEGLRMLVADKFKLNQPDGPSDGWLTQEGLWLVSKPAVDQS
SSTVSGYRTHPHLQRPNV