Protein Info for Psyr_0708 in Pseudomonas syringae pv. syringae B728a

Annotation: Virulence factor MVIN-like protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 528 transmembrane" amino acids 19 to 37 (19 residues), see Phobius details amino acids 43 to 61 (19 residues), see Phobius details amino acids 99 to 127 (29 residues), see Phobius details amino acids 149 to 169 (21 residues), see Phobius details amino acids 177 to 197 (21 residues), see Phobius details amino acids 203 to 221 (19 residues), see Phobius details amino acids 252 to 278 (27 residues), see Phobius details amino acids 290 to 308 (19 residues), see Phobius details amino acids 327 to 348 (22 residues), see Phobius details amino acids 370 to 390 (21 residues), see Phobius details amino acids 400 to 419 (20 residues), see Phobius details amino acids 425 to 443 (19 residues), see Phobius details amino acids 464 to 482 (19 residues), see Phobius details amino acids 495 to 517 (23 residues), see Phobius details TIGR01695: murein biosynthesis integral membrane protein MurJ" amino acids 18 to 519 (502 residues), 550 bits, see alignment E=2.8e-169 PF03023: MurJ" amino acids 44 to 493 (450 residues), 523.4 bits, see alignment E=2.3e-161

Best Hits

Swiss-Prot: 74% identical to MURJ_SALTY: Probable lipid II flippase MurJ (murJ) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K03980, virulence factor (inferred from 100% identity to psb:Psyr_0708)

MetaCyc: 74% identical to lipid II flippase MurJ (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-286

Predicted SEED Role

"Proposed peptidoglycan lipid II flippase MurJ" in subsystem ZZ gjo need homes

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZYJ6 at UniProt or InterPro

Protein Sequence (528 amino acids)

>Psyr_0708 Virulence factor MVIN-like protein (Pseudomonas syringae pv. syringae B728a)
MAGLIAHPLPRRTTLSMNLLKSLAAVSSITMVSRVLGFVRDTIIARTFGAGMATDAFFIA
FKLPNLLRRIFAEGAFSQAFVPILAEYKSQQGEEATRTFISYVTGLLTLALALVTLLGVI
FAPWVIWATAPGFVDTPEKFALTSDLLRVTFPYILLISLSSMAGAILNTWNRFSVPAFVP
TLLNVSMIFFALFLTPYFDPPVMALGWAVLVGGLLQLLYQLPHLKKIGMLVLPRLNLRDT
GVWRVMKQMLPAILGVSVSQISLIINTIFASFLVAGSVSWMYYADRLMELPSGVLGVALG
TILLPILSKTYAQRDRQEYSRILDWGLRLCFVLVLPCTLALGLLAEPLTVSLFQYGKFDA
LDAAMTQRALVAYSVGLLGIILIKVLAPGFYAQQNIRTPVKIAIFTLIVTQLLNLAFIVP
LQHAGLALAISVGACINAGLLFWQLRKQDLFQPQPGWTKFLFKLVIAVAVMSAVLLGLMH
VMPAWDEGHMLERFLRLGALVAAGVVTYFAMLLLLGFRLRDFARKAIM