Protein Info for Psyr_0691 in Pseudomonas syringae pv. syringae B728a
Annotation: gamma-glutamyltransferase 1, Threonine peptidase, MEROPS family T03
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 80% identical to GGT_PSEUA: Glutathione hydrolase proenzyme (ggt) from Pseudomonas sp. (strain A14)
KEGG orthology group: K00681, gamma-glutamyltranspeptidase [EC: 2.3.2.2] (inferred from 100% identity to psb:Psyr_0691)Predicted SEED Role
"Gamma-glutamyltranspeptidase (EC 2.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle or Utilization of glutathione as a sulphur source (EC 2.3.2.2)
MetaCyc Pathways
- γ-glutamyl cycle (4/6 steps found)
- glutathione degradation (DUG pathway) (1/2 steps found)
- leukotriene biosynthesis (2/6 steps found)
- hypoglycin biosynthesis (4/14 steps found)
- peptido-conjugates in tissue regeneration biosynthesis (6/17 steps found)
KEGG Metabolic Maps
- Arachidonic acid metabolism
- Cyanoamino acid metabolism
- Glutathione metabolism
- Selenoamino acid metabolism
- Taurine and hypotaurine metabolism
Isozymes
Compare fitness of predicted isozymes for: 2.3.2.2
Use Curated BLAST to search for 2.3.2.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q4ZYL1 at UniProt or InterPro
Protein Sequence (575 amino acids)
>Psyr_0691 gamma-glutamyltransferase 1, Threonine peptidase, MEROPS family T03 (Pseudomonas syringae pv. syringae B728a) MKFEPLARSLIATALIVAYSPTFAASQAPVAAENGMVVTAQHLATHVGVDVLKAGGNAVD AAVAVGYALAVVYPAAGNLGGGGFMTIQLADGRKTFLDFREKAPLAATANMYLDKDGKVI PDLSAKGHLAVGVPGTVSGMEMALSKYGTRKREEVIAPAIKLAEDGFVLGQGDVDMLSSA TDVFKADMADSGSIFLDKGEPMQVGQKLVQKDLAKTLKEVSEKGSDGFYKGWVAKALVDS SQAGKGIITQADLDHYKTRELAPVECDYRGYHVVSAPPPSSGGVVICQILNILEGYPMKE LGFRSAQGMHYQIEAMRHAYADRNSYLGDPDFVKNPVEHLLDVKYAAKLRAAIDPQKAGD SNKIKPGVAPHEGSNTTHYSIVDKWGNAVSVTYTLNNWFGAGVMASKTGVILNDEMDDFT VKVGVPNMYGLVQGEANAIAPGKTPLSSMSPTIVTKDGKAVMVVGTPGGSRIITATLLTM LNVIDYGMNIQEAVDAPRFHQQWQPEETNIDTFAVSPDTLKILQSWGQKFAGPQPLNHVA AILVGAPSLGGKPVGNNRFYGANDPRRNTGLSLGY