Protein Info for Psyr_0482 in Pseudomonas syringae pv. syringae B728a

Annotation: aspartate carbamoyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00670: aspartate carbamoyltransferase
  • amino acids 19 to 319 (301 residues), 296.9 bits, E=7.4e-93
• PF02729: OTCace_N
  • amino acids 19 to 163 (145 residues), 147 bits, E=4.5e-47
• PF00185: OTCace
  • amino acids 171 to 317 (147 residues), 82.5 bits, E=3.5e-27

Best Hits

Swiss-Prot: 100% identical to PYRB_PSEU2: Aspartate carbamoyltransferase (pyrB) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K00609, aspartate carbamoyltransferase catalytic subunit [EC: 2.1.3.2] (inferred from 99% identity to pst:PSPTO_5040)

Predicted SEED Role

"Aspartate carbamoyltransferase (EC 2.1.3.2)" in subsystem De Novo Pyrimidine Synthesis (EC 2.1.3.2)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)
• UMP biosynthesis III (5/6 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.3.2

Sequence Analysis Tools

See Q4ZZ70 at UniProt or InterPro

Protein Sequence (334 amino acids)

>Psyr_0482 aspartate carbamoyltransferase (Pseudomonas syringae pv. syringae B728a)
MTPLDAKRPLQLNHLGQLRHFLSLDGLPRELLTEILDTADSFLEVGARAVKKVPLLRGKT
VCNVFFENSTRTRTTFELAAQRLSADVITLNVSTSSTSKGETLFDTLRNLEAMAADMFVV
RHADSGAAHFIAEHVCPDVAIINGGDGRHAHPTQGMLDMLTIRRHKGGFENLSVAIVGDI
LHSRVARSNMLALKALGCPDIRVIGPKTLLPVGVEQYGVKVYTDLNEGLKDVDVVIMLRL
QRERMTGGLLPSEGEFYRLFGLTTARLAAAKPDAIVMHPGPINRGVEIESAVADGAHSVI
LNQVTYGIAIRMAVLSMAMSGQNAQRQFEQENAQ