Protein Info for Psyr_0321 in Pseudomonas syringae pv. syringae B728a

Annotation: aminopeptidase P, Metallo peptidase, MEROPS family M24B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 444 PF05195: AMP_N" amino acids 9 to 128 (120 residues), 137 bits, see alignment E=2.7e-44 PF00557: Peptidase_M24" amino acids 183 to 414 (232 residues), 221.3 bits, see alignment E=1.3e-69

Best Hits

KEGG orthology group: K01262, Xaa-Pro aminopeptidase [EC: 3.4.11.9] (inferred from 100% identity to psb:Psyr_0321)

Predicted SEED Role

"Xaa-Pro aminopeptidase (EC 3.4.11.9)" (EC 3.4.11.9)

Isozymes

Compare fitness of predicted isozymes for: 3.4.11.9

Use Curated BLAST to search for 3.4.11.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZZM9 at UniProt or InterPro

Protein Sequence (444 amino acids)

>Psyr_0321 aminopeptidase P, Metallo peptidase, MEROPS family M24B (Pseudomonas syringae pv. syringae B728a)
MIQIPKSEYARRRKALMAQMEPDSIAILPAAAVAIRNRDVEHVYRQDSDFQYLSGFPEPE
AVVVLIPGREYGEYVLFCRERNPERELWEGLRAGQEGAIREYGADDAFPINDIDEILPGL
IEGRDRVYSAMGSNPEFDRHLMDWINVIRSKAHLGAQPPKEFVALDHLLHDMRLYKSAAE
IKVMRCAADISARAHVRAMQACRAGLHEFSLEAELDYEFRKGGSKMPAYGSIVASGRNGC
ILHYQQNDAVLRDGDLVLIDAGCEIDCYASDITRTFPVNGRFSPEQKAIYELVLKSQQAA
FAAIGPDKHWNQAHEATVKVITAGLVELGLLRGDVRELIESEAYKMFYMHRAGHWLGMDV
HDVGEYKVGGEWRVLEVGMTLTVEPGIYISPDNLDVAKKWRGIGVRIEDDVVVTRQGCEI
LSGGVPKTVAEIEALMAAAQEQVV