Protein Info for Psyr_0283 in Pseudomonas syringae pv. syringae B728a

Annotation: NLPA lipoprotein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 257 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details PF03180: Lipoprotein_9" amino acids 22 to 257 (236 residues), 324.1 bits, see alignment E=2.1e-101

Best Hits

Swiss-Prot: 39% identical to TPN32_TREPA: Membrane lipoprotein TpN32 (tpn32) from Treponema pallidum (strain Nichols)

KEGG orthology group: K02073, D-methionine transport system substrate-binding protein (inferred from 100% identity to psb:Psyr_0283)

Predicted SEED Role

"Methionine ABC transporter substrate-binding protein" in subsystem Methionine Biosynthesis or Methionine Degradation or Staphylococcal pathogenicity islands SaPI

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZZR6 at UniProt or InterPro

Protein Sequence (257 amino acids)

>Psyr_0283 NLPA lipoprotein (Pseudomonas syringae pv. syringae B728a)
MKKLLAIFAAVTALSAQANETLTVAASAVPHAEILEFVKPTLAKEGVDLNIKVFNDYIQP
NVQVSQNRMDANFFQHQPYLDEFNKGKGTDLVAVAKVHIEPFGAYSEKFKTLAELPNDAN
VALPNDATNEGRALLLLAKAGLITLKEPGNILSKPSDVVNNPKNLKFRELEAATLPRVLT
QVDLALINTNYALSAKLDPTKDALILEGADSPYANILVARPDNKDSDAMKKLVAALQSPE
VKTFLAEKYKGAVLPAF