Protein Info for Psyr_0077 in Pseudomonas syringae pv. syringae B728a

Annotation: sigma54 specific transcriptional regulator, Fis family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 439 PF08448: PAS_4" amino acids 25 to 126 (102 residues), 70.2 bits, see alignment E=4.2e-23 PF00158: Sigma54_activat" amino acids 140 to 306 (167 residues), 215.8 bits, see alignment E=7.8e-68 PF14532: Sigma54_activ_2" amino acids 141 to 311 (171 residues), 75.1 bits, see alignment E=1.7e-24 PF07728: AAA_5" amino acids 163 to 284 (122 residues), 29.9 bits, see alignment E=1.3e-10 PF02954: HTH_8" amino acids 403 to 434 (32 residues), 33.3 bits, see alignment (E = 7.8e-12)

Best Hits

KEGG orthology group: None (inferred from 100% identity to psb:Psyr_0077)

Predicted SEED Role

"Sigma-54 dependent transcriptional regulator"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q500M1 at UniProt or InterPro

Protein Sequence (439 amino acids)

>Psyr_0077 sigma54 specific transcriptional regulator, Fis family (Pseudomonas syringae pv. syringae B728a)
MNLIATDFPGSRMQSLVSYLEHDLRPTIVLDTDYNIIAANTAYQRQFGVEGTPHVGEKCY
RVSHQFAVPCDQAGEHCPMRKAHETRLPERLLHIHHTPRGPEHVDVELRPILDDQGEVIA
YVERLSSVAVASVQPQQHGLVGRSAAFKEVLSALQRAAPAQIPVLLQGESGTGKELFARA
LHASSARSAGPLVVVDCTGLTETLLESELFGYEKGAFTGALQRKTGLAEAAHGGTLFLDE
IGEVPLAMQVKLLRLIESGSFRPVGSLRTVHSDFRLVVATHKPLREMVADGSFRQDLFYR
ISAFPIRLPALRERSDDLPLLIDSLLQRIAPGAAPRVDAEAMKLLSLYSYPGNIRELRNI
LERARLFTDDGVIRVQDLPAEVRGQGEAVGVTYRQQDDGLEKLAHALRVFNGSRSELARE
LGLSERTLYRRLRALGIPR