Protein Info for Psyr_0003 in Pseudomonas syringae pv. syringae B728a

Annotation: DNA replication and repair protein RecF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 367 TIGR00611: DNA replication and repair protein RecF" amino acids 1 to 359 (359 residues), 388.2 bits, see alignment E=2.1e-120 PF02463: SMC_N" amino acids 3 to 359 (357 residues), 141.2 bits, see alignment E=5.2e-45 PF13476: AAA_23" amino acids 5 to 45 (41 residues), 32.3 bits, see alignment 2.3e-11 PF13304: AAA_21" amino acids 25 to 49 (25 residues), 28.9 bits, see alignment (E = 1.8e-10)

Best Hits

Swiss-Prot: 100% identical to RECF_PSEU2: DNA replication and repair protein RecF (recF) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K03629, DNA replication and repair protein RecF (inferred from 100% identity to psb:Psyr_0003)

Predicted SEED Role

"DNA recombination and repair protein RecF" in subsystem DNA repair, bacterial RecFOR pathway

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q500U5 at UniProt or InterPro

Protein Sequence (367 amino acids)

>Psyr_0003 DNA replication and repair protein RecF (Pseudomonas syringae pv. syringae B728a)
MSLSRVSVTGVRNLHPVTLSPSPRINILYGANGSGKTSVLEAIHLLGIARSFRSSRLLPV
IQYEQPSCTVFGQVDLAQGGHSNLGVSRDRQGEFQIRIDGQNARSAAQLAEILPLQLINP
DSFRLLEGAPKIRRQFLDWGVFHVEPRFMATWQRLQKALKQRNSWLRHGTLDAASQAAWD
RELCSASDEIDEFRRAYIKALKPVFEQTLSELVELEGLTLSYYRGWDKEKELSTVLASSI
HRDQQMGHTQAGPQRADLRLRLGAHNAADILSRGQQKLVVCALRIAQGHLVSQVRRGQCI
YLVDDLPSELDDNHRRALCRLLEELRCQVFITCVDQEFLREGWQTETPVALFHVEQGRIT
QTHDHRE