Protein Info for Pf6N2E2_994 in Pseudomonas fluorescens FW300-N2E2

Annotation: NADH-dependent dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 362 PF01408: GFO_IDH_MocA" amino acids 29 to 128 (100 residues), 44.8 bits, see alignment E=2.9e-15 PF22725: GFO_IDH_MocA_C3" amino acids 137 to 258 (122 residues), 60.5 bits, see alignment E=2.5e-20 PF02894: GFO_IDH_MocA_C" amino acids 143 to 353 (211 residues), 48.3 bits, see alignment E=1.7e-16

Best Hits

KEGG orthology group: None (inferred from 67% identity to lch:Lcho_0838)

Predicted SEED Role

"NADH-dependent dehydrogenase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZV74 at UniProt or InterPro

Protein Sequence (362 amino acids)

>Pf6N2E2_994 NADH-dependent dehydrogenase (Pseudomonas fluorescens FW300-N2E2)
MTTKIDNQTKLGVACLGITHPHTSGRVKAFQRIPGVEFLGAYDDSPLLEPFADALGLQAR
SKEDILSDPNVHIVLVHPKSYLMADWAIEALEAGKAVLCEKPAGRHSTDTLRIVEAVERT
GNLFQVGYCWRFSPAVEKMQQVLQSGEIGKVLQVRAHAGCSHDEADTNHMKQPGDLGGAF
YVIGCHTIDRILLHFGMPKFVNARITKFAGQMSNSAREDAAVAILNYDDKVVTIDFMSWD
PMPWTESWDVTAYGTHGVMHSTPMPASYKLYHDGKIGHSQGWTHWNETSFPEVWAVRKTV
YSPEIAEIGNPVYFDREAAAFVKSLTEGLPSQVPATQAHNINLILEALFKSSELLGQEVS
LI