Protein Info for Pf6N2E2_983 in Pseudomonas fluorescens FW300-N2E2

Annotation: Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 330 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details TIGR03558: luciferase family oxidoreductase, group 1" amino acids 4 to 324 (321 residues), 428.1 bits, see alignment E=1.1e-132 PF00296: Bac_luciferase" amino acids 10 to 295 (286 residues), 139.5 bits, see alignment E=8.3e-45

Best Hits

Swiss-Prot: 52% identical to YHBW_ECOL6: Luciferase-like monooxygenase (yhbW) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K00494, alkanal monooxygenase (FMN-linked) [EC: 1.14.14.3] (inferred from 60% identity to vap:Vapar_0386)

Predicted SEED Role

"Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.14.14.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZU13 at UniProt or InterPro

Protein Sequence (330 amino acids)

>Pf6N2E2_983 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases (Pseudomonas fluorescens FW300-N2E2)
MTALSILDLIPINVGSTLAQTYENSVKLAQHAEACGYRRYWLAEHHNMPGIGSSATTLLM
SHLAANTNSIRIGSGGIMLPNHSPLTVAEQFGTLETLYPGRIDLGLGRAPGADGVTMRAL
RRSTETSADQFPRDVVEILDYFEDNGRQPVRAVPGAGQDIPVWILGSSTYGASLAAHLGL
PYAFASHFAPRLIKEALQVYRSQFQPSHYLDKPYVMAGINVFAAETRAEAEFIASSHRKW
VAGVHTGDITLLPAPVEDYMQGIAPSLRASLDRELAFTAIGTQEDVRSTMMEFIEFTGAD
ELMVDARIFDPAARRKSYEIAAQALADQLA