Protein Info for Pf6N2E2_948 in Pseudomonas fluorescens FW300-N2E2

Annotation: Multidrug efflux transporter MexF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1063 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details transmembrane" amino acids 28 to 30 (3 residues), see Phobius details amino acids 342 to 362 (21 residues), see Phobius details amino acids 369 to 389 (21 residues), see Phobius details amino acids 395 to 416 (22 residues), see Phobius details amino acids 440 to 462 (23 residues), see Phobius details amino acids 473 to 499 (27 residues), see Phobius details amino acids 546 to 565 (20 residues), see Phobius details amino acids 881 to 901 (21 residues), see Phobius details amino acids 908 to 928 (21 residues), see Phobius details amino acids 934 to 958 (25 residues), see Phobius details amino acids 982 to 1001 (20 residues), see Phobius details amino acids 1011 to 1037 (27 residues), see Phobius details PF00873: ACR_tran" amino acids 3 to 1038 (1036 residues), 1251.7 bits, see alignment E=0 TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 3 to 1052 (1050 residues), 1192.1 bits, see alignment E=0 PF02355: SecD_SecF_C" amino acids 341 to 498 (158 residues), 27.6 bits, see alignment E=2.7e-10 PF03176: MMPL" amino acids 346 to 501 (156 residues), 43.2 bits, see alignment E=3.8e-15 amino acids 881 to 1045 (165 residues), 25.6 bits, see alignment E=9e-10

Best Hits

KEGG orthology group: None (inferred from 80% identity to avn:Avin_33870)

Predicted SEED Role

"Multidrug efflux transporter MexF"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165YXQ5 at UniProt or InterPro

Protein Sequence (1063 amino acids)

>Pf6N2E2_948 Multidrug efflux transporter MexF (Pseudomonas fluorescens FW300-N2E2)
MKFSQFFITRPIFAAVLSLLILIAGAISLFQLPISEYPEVVPPTVVVHANYPGANPKVIG
ETVAAPLEQAITGVENMLYMSSQSTADGRLTLTVTFALGTDLDNAQVQVQNRVTRTEPKL
PEEVTRIGITVDKASPELTLLVHLVSPDKRYDMLYLSNYAILNVKDELSRLDGIGDVKMF
GIGDYSLRIWLDPNKTASRNLTATDVVNAVREQNRQVAAGQLGSPPAPNATSFQMSINSQ
GRLVSEEEFENVIIRSGPDGEITRLKDIGRVELGSNQYALRALLNNEEAVAMPIFQRPGS
NAIDISNQVRAKMAELKKSFPEGMDYEIVYDPTIFVRSSIEAVVHTLFEALILVVLVVIL
FLQTWRASIIPLVAVPVSLIGTFAVMHLFGFSLNALSLFGLVLAIGIVVDDAIVVVENVE
RNIESGLEPVEATKKAMGEVTGPIIATALVLCAVFVPAAFISGLTGQFYKQFALTIAIST
VISAFNSLTLSPALAAVLLKAHGAPKDRFSKVLDRLLGGWLFKPFNRFFDKASHGYVIAV
AKVIRGSSVALLVYAGLIAMTYVGFSTTPTGFVPTQDKKYLVTFAQLPDAASLDRTESVI
RRMSTIAMNEPGFDSAVAFPGLSINGFTNSSNAGIAFIGLRPFDERKDPSLSAGAISASL
NAKFGGIQDAYIAVFPPPPVQGLGTIGGFRLQIEDRGNLGYEELYKETMNIIGKSRSVPE
LANLFTSYTVNVPQVDAAIDREKAKIHGVAINDIFDTLQVYLGSLYANDFNRFGRTYQVN
VQAEQQFRQEPEQIGQLKVRNNKGEMIPLATFVKVSPTSGPDRVMHYNGFITAEINGGAA
PGYSSGQAQAAIEKLLKEELPNGMTYEWTDLTYQQILSGNTALLVFPLCVLLAFLVLAAL
YESWSLPLAVILIVPMTLLSAIAGVIISKGDNNIFTQIGLIVLVGLACKNAILIVEFAKD
KQAQGMSPLEAVLDACRMRLRPILMTSFAFIMGVIPLVTSTGAGSEMRRAMGVAVFSGML
GVTFFGLLLTPVFFVLIRRSIERNNARKAARINQTHSLNSEAH