Protein Info for Pf6N2E2_897 in Pseudomonas fluorescens FW300-N2E2

Annotation: Maleate cis-trans isomerase (EC 5.2.1.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 75% identical to MAIA_ALCFA: Maleate isomerase (maiA) from Alcaligenes faecalis

KEGG orthology group: K01799, maleate isomerase [EC: 5.2.1.1] (inferred from 78% identity to rpf:Rpic12D_1552)

MetaCyc: 75% identical to maleate isomerase monomer (Alcaligenes faecalis)
Maleate isomerase. [EC: 5.2.1.1]

Predicted SEED Role

"Maleate cis-trans isomerase (EC 5.2.1.1)" in subsystem Salicylate and gentisate catabolism (EC 5.2.1.1)

MetaCyc Pathways

nicotinate degradation II (1/5 steps found)
nicotinate degradation I (1/6 steps found)
picolinate degradation (1/7 steps found)
nicotine degradation II (pyrrolidine pathway) (3/11 steps found)
nicotine degradation III (VPP pathway) (2/10 steps found)
superpathway of nicotinate degradation (1/18 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.2.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZS83 at UniProt or InterPro

Protein Sequence (250 amino acids)

>Pf6N2E2_897 Maleate cis-trans isomerase (EC 5.2.1.1) (Pseudomonas fluorescens FW300-N2E2)
MKNFRIGQIVPSSNTTMETEIPAMLTSRYELFPEEHFTFHSSRMRMMHVSPEELKKMDID
SDRCALELSDARVDVMAYACLVAIMCQGAGYHKVSQERLSKTVASNQSTAPVLSSAGALI
DSLNMLDYKKVSIITPYMKPLTRQVIDYIEAAGIEVVDSISLEVSDNLEVGRLDPMNLLA
HADRLNIGQADGVVLSCCVQMPSLPAIQLVQDRLDKPVLSASVATVYQMLKTLGLKAQVP
NAGHLLSGAF