Protein Info for Pf6N2E2_876 in Pseudomonas fluorescens FW300-N2E2
Annotation: Cobalt-containing nitrile hydratase subunit beta (EC 4.2.1.84)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01721, nitrile hydratase [EC: 4.2.1.84] (inferred from 72% identity to kpe:KPK_2673)Predicted SEED Role
"Cobalt-containing nitrile hydratase subunit beta (EC 4.2.1.84)" (EC 4.2.1.84)
MetaCyc Pathways
- aldoxime degradation (3/3 steps found)
- acrylonitrile degradation I (2/2 steps found)
- indole-3-acetate biosynthesis IV (bacteria) (2/2 steps found)
- superpathway of acrylonitrile degradation (2/3 steps found)
- indole-3-acetate biosynthesis II (5/12 steps found)
KEGG Metabolic Maps
- 1,4-Dichlorobenzene degradation
- Benzoate degradation via CoA ligation
- Cyanoamino acid metabolism
- Styrene degradation
- Tryptophan metabolism
Isozymes
Compare fitness of predicted isozymes for: 4.2.1.84
Use Curated BLAST to search for 4.2.1.84
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A159ZW19 at UniProt or InterPro
Protein Sequence (217 amino acids)
>Pf6N2E2_876 Cobalt-containing nitrile hydratase subunit beta (EC 4.2.1.84) (Pseudomonas fluorescens FW300-N2E2) MNGVHDLGGMHGFGPVLTEENEPLFHHDWERRVFPLFASLFVGGHFNVDEFRHAIERMEP AEYLQSSYYEHWLHAFENLLLEKGVISTGELQGTAKPTPPTQPVTVLTPDIVEAVVLGGA SSRAKEPVSGRFQVGDRVRTKNLNPTTHTRLPRYARGKVGTIEIAHGAFATPDTMAHGLG EQPQQVYAVRFSATELWGVARPDSVCIDLWDSYLEAV