Protein Info for Pf6N2E2_839 in Pseudomonas fluorescens FW300-N2E2

Annotation: Alkaline phosphatase (EC 3.1.3.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 873 PF00413: Peptidase_M10" amino acids 71 to 172 (102 residues), 26.4 bits, see alignment E=1.4e-09 PF13583: Reprolysin_4" amino acids 150 to 214 (65 residues), 28.8 bits, see alignment 2.4e-10 PF08548: Peptidase_M10_C" amino acids 214 to 349 (136 residues), 50.2 bits, see alignment E=7.2e-17 amino acids 677 to 872 (196 residues), 29 bits, see alignment E=2.2e-10 PF00353: HemolysinCabind" amino acids 301 to 335 (35 residues), 28.4 bits, see alignment (E = 3.2e-10) amino acids 384 to 418 (35 residues), 22.1 bits, see alignment (E = 2.9e-08) amino acids 467 to 501 (35 residues), 30.1 bits, see alignment (E = 9.1e-11) amino acids 551 to 585 (35 residues), 31.2 bits, see alignment (E = 4.3e-11) amino acids 577 to 593 (17 residues), 12 bits, see alignment (E = 4.5e-05) amino acids 643 to 677 (35 residues), 31.2 bits, see alignment (E = 4.3e-11) amino acids 669 to 685 (17 residues), 12 bits, see alignment (E = 4.5e-05) amino acids 735 to 769 (35 residues), 30.1 bits, see alignment (E = 9.3e-11) amino acids 755 to 778 (24 residues), 19.4 bits, see alignment (E = 2.1e-07)

Best Hits

Predicted SEED Role

"Alkaline phosphatase (EC 3.1.3.1)" in subsystem Phosphate metabolism (EC 3.1.3.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.1

Use Curated BLAST to search for 3.1.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165YWX0 at UniProt or InterPro

Protein Sequence (873 amino acids)

>Pf6N2E2_839 Alkaline phosphatase (EC 3.1.3.1) (Pseudomonas fluorescens FW300-N2E2)
MPTPVGYSYTSSATLTGNAQVDSLLVGTYWMGNTWSTGTFTTPLTYSFMTETSRFARYYS
SDNEYEASYVLTSAQQSGITSALDLWGSVANIKFTQVSESTTTVGDLRFGGYLNMESDTA
AWGYFPADTPSGGDVWLGPVTNNPTPDKGTYDYLVFMHEIGHALGLKHPFSQSLSNTTVL
AAQFDDVRNTIMSYNTSYSYEPTTPMVLDIAAIQSLYGANTQWQTGNNTYRWEPSQSVFE
TIWDAGGIDTIDASNQLAAVRINLNEGQFSKIGQSFLELDTQTAFSEGLAIAYGAKIENA
FGSANDDTLIGNALANVLNGRAGEDTMIGGAGNDTYVVDNIGDSVQESSTLASEIDAVIS
SVSYVLGANIETLRLGASANINGTGNTLNNRIDGNAGANTLDGGSGIDNLTGGAGNDTYV
VDNLKDVVSETSALASEIDTVRSSVSWTLGANVENLILAGSAPINGIGNALNNVLTGNAA
NNVLNGGVGQDTLIGGAGNDTYLVDDLKDIVSETSTLAGETDTVRSSLKWSLGANLENLT
LTGSAHLNGGGNALNNVLIGNDGNNVLSGWGGSDTLDGGSGIDTLVGGTGNDTYVVDNLK
DIVSETSTLAGEIDTVRSSLKWSLGANLENLTLTGSAHLNGGGNALNNVLIGNDGNNVLS
GWGGSDTLDGGSGIDTLVGGTGNDTYVVDNLKDIVSETSTLAGEIDTVRSSLKWSLGANL
ENLTLTGSAHLKGGGNALDNVLIGNDGNNVLSGWGGNDTLSGGLGIDSLTGGAGSDRFVF
NTLGELGMGSTRDTISDFSSLQGDKLDLSKLDANLLTSFADTFSFVNSNDFTAAGQLRFV
DQVLYGNVNGDLGADFEIQLVGVNTLNVTDLIA