Protein Info for Pf6N2E2_824 in Pseudomonas fluorescens FW300-N2E2

Annotation: Multidrug efflux transporter MexF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1059 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details transmembrane" amino acids 342 to 362 (21 residues), see Phobius details amino acids 369 to 389 (21 residues), see Phobius details amino acids 395 to 416 (22 residues), see Phobius details amino acids 440 to 462 (23 residues), see Phobius details amino acids 472 to 499 (28 residues), see Phobius details amino acids 548 to 567 (20 residues), see Phobius details amino acids 881 to 899 (19 residues), see Phobius details amino acids 906 to 928 (23 residues), see Phobius details amino acids 934 to 957 (24 residues), see Phobius details amino acids 982 to 1002 (21 residues), see Phobius details amino acids 1011 to 1037 (27 residues), see Phobius details TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 3 to 1049 (1047 residues), 1175.3 bits, see alignment E=0 PF00873: ACR_tran" amino acids 3 to 1038 (1036 residues), 1265.8 bits, see alignment E=0 PF02355: SecD_SecF_C" amino acids 342 to 498 (157 residues), 24.9 bits, see alignment E=1.8e-09 PF03176: MMPL" amino acids 350 to 498 (149 residues), 29.1 bits, see alignment E=7.4e-11 amino acids 887 to 1046 (160 residues), 21.9 bits, see alignment E=1.2e-08

Best Hits

KEGG orthology group: None (inferred from 82% identity to avn:Avin_33870)

Predicted SEED Role

"Multidrug efflux transporter MexF"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZVX8 at UniProt or InterPro

Protein Sequence (1059 amino acids)

>Pf6N2E2_824 Multidrug efflux transporter MexF (Pseudomonas fluorescens FW300-N2E2)
MKFSQFFISRPIFAAVLSLLILIAGAISLFQLPISEYPEVVPPTVVVRANFPGANPKVIG
ETVAAPLEQAITGVENMLYMSSQSTADGKITLTITFALGTDLDNAQVQVQNRVTRTEPKL
PEEVTRIGITVDKASPDLTMVVHLTSPDKRYDMLYLSNYALLNIKDELARLDGIGDVQLF
GMGDYSLRVWLDPNKTASRNLTATDVVTAIREQNRQVAAGALGAPPAPNAQAFQLSINTQ
GRLVNEEEFENIIIRSGANGEITRLKDIARVELGSSQYALRSLLDNQPAVAIPIFQRPGS
NAIQISNDVRAKMEELKKGFPAGMDYSIVYDPTIFVRGSIEAVVHTLFEALILVVLVVIL
FLQTWRASIIPLVAVPVSLIGTFAVMHLFGFSLNALSLFGLVLAIGIVVDDAIVVVENVE
RNIELGLTPVEATKRAMGEVTGPIIATALVLCAVFVPAAFISGLTGQFYKQFALTIAIST
VISAINSLTLSPALAAVLLKGHDAPKDRFSKFLDKVFGGWLFRPFNRFFERASHGYVGTV
ARVIRSSGIALVLYAGLMVLTFFGFSTTPTGFVPGQDKQYLVAFAQLPDAASLDRTEDVI
KRMSDLALKQPGVESAVAFPGLSINGFTNSPNAGIVFVTLKPFDERKDPSMSAGAIAGAL
NGQYAGIQEAYMAIFPPPPVQGLGTIGGFRLQIEDRGNLGYDELYKETMNIITKSRSVPE
LAGLFTSYTVNVPQVDAAIDREKAKTHGVAVSDIFDTLQIYLGSLYANDFNRFGRTYQVN
VQAEQQFRLESDQIGQLKVRNNRGEMIPLATFIKVSDTSGPDRVMHYNGFITAEINGAAA
PGYSSGQAEKAIEKLLKDELPNGMTYEWTDLTYQQILSGNTALFVFPLCVLLAFLVLAAQ
YESWSLPLAVILIVPMTLLSAITGVILSGGDNNIFTQIGLIVLVGLACKNAILIVEFAKD
KQLEGMNPLAAVLEACRLRLRPILMTSFAFIMGVVPLVFSSGAGAEMRHAMGVAVFSGML
GVTFFGLLLTPVFYVLIRNFVERSEARKAARALKLEAQQ