Protein Info for Pf6N2E2_6083 in Pseudomonas fluorescens FW300-N2E2

Annotation: Threonine and homoserine efflux system

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 296 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details transmembrane" amino acids 40 to 61 (22 residues), see Phobius details amino acids 73 to 92 (20 residues), see Phobius details amino acids 98 to 117 (20 residues), see Phobius details amino acids 124 to 143 (20 residues), see Phobius details amino acids 149 to 168 (20 residues), see Phobius details amino acids 180 to 202 (23 residues), see Phobius details amino acids 208 to 227 (20 residues), see Phobius details amino acids 239 to 259 (21 residues), see Phobius details amino acids 265 to 284 (20 residues), see Phobius details PF00892: EamA" amino acids 150 to 280 (131 residues), 60.3 bits, see alignment E=1.2e-20

Best Hits

Swiss-Prot: 58% identical to RHTA_ECO57: Threonine/homoserine exporter RhtA (rhtA) from Escherichia coli O157:H7

KEGG orthology group: None (inferred from 99% identity to pba:PSEBR_a3937)

MetaCyc: 58% identical to L-threonine/L-homoserine exporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-242; TRANS-RXN0-0244

Predicted SEED Role

"Threonine and homoserine efflux system"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161GUC7 at UniProt or InterPro

Protein Sequence (296 amino acids)

>Pf6N2E2_6083 Threonine and homoserine efflux system (Pseudomonas fluorescens FW300-N2E2)
MTPPPHRSLASTLYPVALLLIAMASIQSGASLAKSMFPVVGAQGTTTLRLIFASLIMLLL
LRPWRAKLTAQSLRTVIVYGMALGGMNFLFYMSVRTVPLGIAVALEFTGPLAVAIYASRR
AIDFLWIALAVAGLLLLIPTGATNAGIDLLGAGYALGAGVCWALYILFGQKAGADNGVQT
AALGVMIAALFVAPIGIVHAGAALLTPSLIPIAIGVAILSTALPYTLEMISLTRMPARTF
GTLMSVEPAVAALSGLVFLQEYLSLAQWLAITCIILASVGATMTMGNTAKPAVAAD