Protein Info for Pf6N2E2_5969 in Pseudomonas fluorescens FW300-N2E2

Updated annotation (from data): ABC transporter for D-galactose/L-arabinose, ATPase component
Rationale: Specifically important for utilizing D-galactose and L-arabinose
Original annotation: L-arabinose transport ATP-binding protein AraG (TC 3.A.1.2.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 514 PF00005: ABC_tran" amino acids 31 to 180 (150 residues), 103.1 bits, see alignment E=2e-33 amino acids 282 to 435 (154 residues), 67.4 bits, see alignment E=2.1e-22

Best Hits

Swiss-Prot: 87% identical to ARAG_PSEPF: Arabinose import ATP-binding protein AraG (araG) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K10539, L-arabinose transport system ATP-binding protein [EC: 3.6.3.17] (inferred from 99% identity to pba:PSEBR_a4034)

MetaCyc: 59% identical to arabinose ABC transporter ATP binding subunit (Escherichia coli K-12 substr. MG1655)
ABC-2-RXN [EC: 7.5.2.12, 7.5.2.13]

Predicted SEED Role

"L-arabinose transport ATP-binding protein AraG (TC 3.A.1.2.2)" in subsystem L-Arabinose utilization (TC 3.A.1.2.2)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.17

Use Curated BLAST to search for 3.6.3.17 or 7.5.2.12 or 7.5.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165ZSX8 at UniProt or InterPro

Protein Sequence (514 amino acids)

>Pf6N2E2_5969 ABC transporter for D-galactose/L-arabinose, ATPase component (Pseudomonas fluorescens FW300-N2E2)
MQAQTATRQHNIGGSLRFNGIGKSFPGVQALANISFVAHPGQVHALMGENGAGKSTLLKI
LGGAYIPSSGDLQIGEQTMAFKGTADSIASGVAVIHQELHLVPEMTVAENLFLGHLPARF
GLVNRGVLRQQALTLLKGLADEIDPQEKVGRLSLGQRQLVEIAKALSRGAHVIAFDEPTS
SLSAREIDRLMAIIGRLRDEGKVVLYVSHRMEEVFRICNAVTVFKDGRYVRTFENMSELT
HDQLVTCMVGRDIQDIYDYRPRERGDVALQVKGLLGPGLHEPVSFQVHKGEILGLFGLVG
AGRTELLRLLSGLERQREGSLVLHDKELKLRSPRDAIAAGVLLCPEDRKKEGIIPLGSVG
ENINISARPSHSTLGCLLRGDWERGNADKQIKSLKVKTPTAGQKIMYLSGGNQQKAILGR
WLSMPMKVLLLDEPTRGIDIGAKAEIYQIIHNLAADGIAVIVVSSDLMEVMGISDRILVL
CEGAMRGELSRDQANESNLLQLALPRQRVADAAN