Protein Info for Pf6N2E2_5942 in Pseudomonas fluorescens FW300-N2E2

Annotation: Ribonuclease E (EC 3.1.26.12)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1065 TIGR00757: ribonuclease, Rne/Rng family" amino acids 10 to 419 (410 residues), 555.2 bits, see alignment E=4.6e-171 PF00575: S1" amino acids 33 to 112 (80 residues), 47.4 bits, see alignment 3.2e-16 PF10150: RNase_E_G" amino acids 116 to 385 (270 residues), 337.5 bits, see alignment E=9.4e-105 PF20833: RNase_E_G_Thio" amino acids 397 to 481 (85 residues), 111.8 bits, see alignment E=2.1e-36

Best Hits

KEGG orthology group: K08300, ribonuclease E [EC: 3.1.26.12] (inferred from 97% identity to pba:PSEBR_a4059)

Predicted SEED Role

"Ribonuclease E (EC 3.1.26.12)" in subsystem RNA processing and degradation, bacterial or Ribosome biogenesis bacterial (EC 3.1.26.12)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.26.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161H536 at UniProt or InterPro

Protein Sequence (1065 amino acids)

>Pf6N2E2_5942 Ribonuclease E (EC 3.1.26.12) (Pseudomonas fluorescens FW300-N2E2)
MLINATQPEELRVALVDGQRLYDLDIESGAREQKKANIYKGRITRIEPSLEAAFVDFGSE
RHGFLPLKEISREYFKKAPEGRVNIKDVLSEGQEVIVQVEKEERGNKGAALTTFISLAGR
YLVLMPNNPRAGGISRRIEGEERNELREALNGLVAPADMGLIVRTAGLGRSSEEMQWDLD
YLLQLWTAIKEASLDRSAPFLIYQESNVIIRAIRDYLRQDIGEVLIDSVEAQDEALTFIR
QVMPQYASKIKLYEDSVPLFNRFQIESQIETAFQRVVELPSGGSIVIDPTEALVSIDINS
ARATKGSDIEETALQTNLEAAEEIARQLRLRDIGGLIVIDFIDMTPAKNQRAVEEKVREC
LEADRARVQVGRISRFGLLEMSRQRLRPSLGESSGIVCPRCNGTGIIRDVESLSLAILRL
IEEEALKDRTAEVRAQVPIPVAAFLLNEKRNSITKIELRTRARIVILPNDHLETPHFEVQ
RLRDDSPEAHTNQSSYEIAAAAAEVEEVQPAAATRTLVRQEAAVKTAPARANAPVPTEVT
APVAAPAAAPEPSLFKGLVKSLVSLFATKEEPAAPAVVAKPATTERPARNEERRNGRQQT
RNRNGRRDEERKPREERAPREERAPREPREERQPREAREEAPAVAREERAPRPPREERQP
RAPREDRKPRGEREERVRELREPLDAAPAAAATAEERPARQPREERAPRPPREERQPRAE
QAAAAASEEEVLNTEEQLQEDGQDTAEGDRPRRRSRGQRRRSNRRERQRDANGNVIEGSE
ESESTENTEAPSAADLAAGLAVTAAVASSAISAPAEAQAHEQAERATAAVESAPVAEAPV
VEAPVVEATTPVEVTASPEVEVAPAHEAQVAETEVVAEPAPVVEQPVVAAEPAVETPAEE
KAPEPAREEQTAFNWTAEPAAPAPVVEPEPTPEPVKAVEPEVVESAPVVEAPVVAEAPAP
VEVPAPASALTANGRAPNDPREVRRRKREAERLQKEAEQAAAAAAQAPAAEPAPAVEEVA
EAPAPVAETPVESAPTFDEPQHSAEEVVPRHTEALEKEHEPKPHA