Protein Info for Pf6N2E2_5930 in Pseudomonas fluorescens FW300-N2E2

Annotation: Predicted hydrolase of the metallo-beta-lactamase superfamily, clustered with KDO2-Lipid A biosynthesis genes

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 748 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details transmembrane" amino acids 43 to 63 (21 residues), see Phobius details amino acids 230 to 249 (20 residues), see Phobius details amino acids 256 to 277 (22 residues), see Phobius details amino acids 304 to 321 (18 residues), see Phobius details amino acids 327 to 346 (20 residues), see Phobius details amino acids 358 to 378 (21 residues), see Phobius details amino acids 386 to 408 (23 residues), see Phobius details amino acids 415 to 434 (20 residues), see Phobius details amino acids 440 to 462 (23 residues), see Phobius details PF13567: DUF4131" amino acids 22 to 166 (145 residues), 103.6 bits, see alignment E=1.4e-33 TIGR00361: DNA internalization-related competence protein ComEC/Rec2" amino acids 122 to 714 (593 residues), 328.3 bits, see alignment E=8e-102 PF03772: Competence" amino acids 198 to 464 (267 residues), 196.4 bits, see alignment E=9.7e-62 TIGR00360: ComEC/Rec2-related protein" amino acids 219 to 398 (180 residues), 84.1 bits, see alignment E=1.4e-27 PF00753: Lactamase_B" amino acids 498 to 676 (179 residues), 63.1 bits, see alignment E=5.3e-21

Best Hits

KEGG orthology group: K02238, competence protein ComEC (inferred from 96% identity to pba:PSEBR_a4071)

Predicted SEED Role

"Predicted hydrolase of the metallo-beta-lactamase superfamily, clustered with KDO2-Lipid A biosynthesis genes" in subsystem KDO2-Lipid A biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165ZSE6 at UniProt or InterPro

Protein Sequence (748 amino acids)

>Pf6N2E2_5930 Predicted hydrolase of the metallo-beta-lactamase superfamily, clustered with KDO2-Lipid A biosynthesis genes (Pseudomonas fluorescens FW300-N2E2)
MRTGLVALALGLLAPIFLPALPPLWLIAAMPVLALMALPFRTYPLGFFLFGLAWACLSAQ
FALDDRLPIALDGETRWVEGRVVGLPQQGEGVVRFELTDTHSRRTRLPTTMRLAWFGGPP
VNSGERWRLAVKLKRPAGLLNPHGFDYAAWLLSRGVGATGTVKDGHLLHAVRGAWRDEVR
QALAQVDAHGRSGALAALVLGDGGGLSREDWQVLQDTGTVHLLVISGQHIGLLAGLVYLL
VAGAARYGLWPAGLAWLPWACALAFCAALGYGLLAGFEVPVRRACAMIGLVLLWRLRFKQ
PDPWWAWLLAFVGVLVFDPLASLRPGFWLSFAAVAILMFTFGARLGPWRWWQTWTRAQWL
VALGLCPLLLIVGLPVSLSGPLVNLLAVPWISLLVLPSALLGTALLPVPFMGEGLLWIAG
GLLDLLFKGLTLVAGRMPAWVPAAVPPWALAIAVLGAFLLLLPKGVPQRVLGWPMVLLMV
FPPQEHLPQGHAEIWQLDVGQGLAVLVRTRRHTLLYDAGPRFGEADAGERVVLPTLRKLG
VRSLDLMLLSHADADHAGGARAVRKGLPTLEVISGDPDALPAELLAGPCDSGRRWEWDGV
GFELWRWASAVESNQKSCVLLVEAGGERLLLTGDIDTHAERALLDEALAVPIQWLAAPHH
GSRSSSSMALLSRLKPHSVLISRGQGNAFGHPHPQVMARYRRSGLTIYDSAEQGAIRLQL
GAFERPRTEAGHRRYWRDPPAVGAPGQY