Protein Info for Pf6N2E2_5821 in Pseudomonas fluorescens FW300-N2E2

Annotation: Hydrolase, alpha/beta fold family functionally coupled to Phosphoribulokinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 320 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF12146: Hydrolase_4" amino acids 79 to 280 (202 residues), 49.8 bits, see alignment E=2.9e-17 PF12697: Abhydrolase_6" amino acids 80 to 282 (203 residues), 40.3 bits, see alignment E=5.9e-14

Best Hits

KEGG orthology group: K06889, (no description) (inferred from 96% identity to pba:PSEBR_a4172)

Predicted SEED Role

"Hydrolase, alpha/beta fold family functionally coupled to Phosphoribulokinase" in subsystem cAMP signaling in bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165ZR07 at UniProt or InterPro

Protein Sequence (320 amino acids)

>Pf6N2E2_5821 Hydrolase, alpha/beta fold family functionally coupled to Phosphoribulokinase (Pseudomonas fluorescens FW300-N2E2)
MLKLLALSLTLFTGLLSGVAQATVLQRPISLDTGNGELFGSLLLPKSDTPVPVVLIISGS
GPTDRDGNNPDGGRNDSLKRLAWVLARHNIASVRYDKRGVAASLAATPDERNLSVEAYVA
DAVAWSHKLAADPRLGPLILLGHSEGALIASLAAPQTNAAAVISLSGSARPIDQVLRQQL
SNRLPPPLMLRSNELLDSLKAGRPDDNVPAQLLVIFRPSVQPYLISLFRQDPARAFATLK
MPALIIQGSNDIQVSVDDARQLKAAKPDAELALIEGMNHVMRIVPNDVKRQLASYKDPNL
PLAAELGTRLLRFIDSLAAR