Protein Info for Pf6N2E2_5662 in Pseudomonas fluorescens FW300-N2E2

Updated annotation (from data): L-Arginine ABC transporter, permease protein AotM
Rationale: Specific phenotypes on L-Arginine; L-Arginine. (SEED_correct)
Original annotation: Arginine/ornithine ABC transporter, permease protein AotM

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 232 transmembrane" amino acids 16 to 43 (28 residues), see Phobius details amino acids 55 to 78 (24 residues), see Phobius details amino acids 89 to 111 (23 residues), see Phobius details amino acids 162 to 183 (22 residues), see Phobius details amino acids 195 to 216 (22 residues), see Phobius details TIGR01726: amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family" amino acids 12 to 117 (106 residues), 75.3 bits, see alignment E=2.2e-25 PF00528: BPD_transp_1" amino acids 31 to 223 (193 residues), 64.7 bits, see alignment E=4.6e-22

Best Hits

Swiss-Prot: 47% identical to NOCM_AGRFC: Nopaline transport system permease protein NocM (nocM) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: None (inferred from 99% identity to pba:PSEBR_a4322)

MetaCyc: 36% identical to L-arginine ABC transporter membrane subunit ArtM (Escherichia coli K-12 substr. MG1655)
ABC-4-RXN [EC: 7.4.2.1]

Predicted SEED Role

"Arginine/ornithine ABC transporter, permease protein AotM" in subsystem Arginine and Ornithine Degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A563 at UniProt or InterPro

Protein Sequence (232 amino acids)

>Pf6N2E2_5662 L-Arginine ABC transporter, permease protein AotM (Pseudomonas fluorescens FW300-N2E2)
MIFDYNVIYEALPLYFSGLLTTLKLLALSLFFGLLAALPLGLMRVSKQPIVNMTAWLYTY
VIRGTPMLVQLFLIYYGLAQFAIVRESFLWPWLSSATFCACLAFAINTSAYTAEIIAGSL
RATSNGEIEAAKAMGMSRYKLYRRILLPSALRRALPQYSNEVIMMLQTTSLASIVTLIDI
TGAARTVNAQYYLPFEAYITAGVFYLCLTFILVRLFKLAERRWLGYLAPRKH