Protein Info for Pf6N2E2_5597 in Pseudomonas fluorescens FW300-N2E2

Annotation: Electron transport complex protein RnfD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 328 transmembrane" amino acids 14 to 32 (19 residues), see Phobius details amino acids 38 to 58 (21 residues), see Phobius details amino acids 73 to 103 (31 residues), see Phobius details amino acids 115 to 134 (20 residues), see Phobius details amino acids 218 to 239 (22 residues), see Phobius details amino acids 246 to 264 (19 residues), see Phobius details amino acids 275 to 294 (20 residues), see Phobius details amino acids 300 to 319 (20 residues), see Phobius details TIGR01946: electron transport complex, RnfABCDGE type, D subunit" amino acids 9 to 322 (314 residues), 359.5 bits, see alignment E=9.4e-112 PF03116: NQR2_RnfD_RnfE" amino acids 9 to 322 (314 residues), 311.5 bits, see alignment E=3.2e-97

Best Hits

Swiss-Prot: 62% identical to RNFD_PSEMY: Ion-translocating oxidoreductase complex subunit D (rnfD) from Pseudomonas mendocina (strain ymp)

KEGG orthology group: K03614, electron transport complex protein RnfD (inferred from 96% identity to pba:PSEBR_a4383)

Predicted SEED Role

"Electron transport complex protein RnfD" in subsystem Na(+)-translocating NADH-quinone oxidoreductase and rnf-like group of electron transport complexes

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A5R8 at UniProt or InterPro

Protein Sequence (328 amino acids)

>Pf6N2E2_5597 Electron transport complex protein RnfD (Pseudomonas fluorescens FW300-N2E2)
MPPLEPVDERLRQAMTRVLLATLPGVLALLWFQGWGVALNLLLSGVTALAVEALVLRLRR
QPLRRDLNDGSALVSATLLALALPAYCPWWLTVTAAACALLFGKHLWGGVGSNPFNPAML
GYALVLVAFPSQMSHWPALHGMGLSEGLQQVFGAHPAPDAWAGATALDSLRINASLTIDE
LFANDPAFGRFGGRGGEWVNLAFLAGGLFLLQQRVFSWHAPVGMLLSLSVVSLLCWNGSG
SDSHGSPLFHLFSGATMLGAFFIATEPVSGAKAPLARLLFGAGMGLLVYLIRTWGGYPDG
VAFAVLLMNLGVPALDRFAEARQRRPLT