Protein Info for Pf6N2E2_5432 in Pseudomonas fluorescens FW300-N2E2

Annotation: Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1298 TIGR01735: phosphoribosylformylglycinamidine synthase" amino acids 3 to 1297 (1295 residues), 2143.9 bits, see alignment E=0 PF18076: FGAR-AT_N" amino acids 35 to 148 (114 residues), 133.9 bits, see alignment E=6.7e-43 PF18072: FGAR-AT_linker" amino acids 169 to 218 (50 residues), 83.3 bits, see alignment (E = 3.4e-27) PF02769: AIRS_C" amino acids 430 to 587 (158 residues), 116.8 bits, see alignment E=2.6e-37 amino acids 838 to 969 (132 residues), 59 bits, see alignment E=1.7e-19 PF22689: FGAR-AT_PurM_N-like" amino acids 646 to 805 (160 residues), 281.6 bits, see alignment E=4e-88 PF13507: GATase_5" amino acids 1042 to 1297 (256 residues), 366.4 bits, see alignment E=1.7e-113

Best Hits

Swiss-Prot: 66% identical to PUR4_HAHCH: Phosphoribosylformylglycinamidine synthase (purL) from Hahella chejuensis (strain KCTC 2396)

KEGG orthology group: K01952, phosphoribosylformylglycinamidine synthase [EC: 6.3.5.3] (inferred from 64% identity to abo:ABO_0785)

MetaCyc: 65% identical to phosphoribosylformylglycinamide synthetase (Escherichia coli K-12 substr. MG1655)
Phosphoribosylformylglycinamidine synthase. [EC: 6.3.5.3]

Predicted SEED Role

"Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3)" in subsystem De Novo Purine Biosynthesis (EC 6.3.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A1W3 at UniProt or InterPro

Protein Sequence (1298 amino acids)

>Pf6N2E2_5432 Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3) (Pseudomonas fluorescens FW300-N2E2)
MLILRGAPALSAFRHSKLLEQLSQKVPAVSGLYAEFAHFAEVTGGLTGDEQQVLARLLKY
GPSVPVQEPAGRLFLVLPRFGTISPWSSKASDIARNCGLAKVQRLERGIAFYVAGEFSEA
EAQLIADGLHDRMTQVVLGNLEQAAGLFSHAEPKPLTAIDVLGGGRAALEKANAELGLAL
AEDEIDYLVNAFQGLKRNPHDIELMMFAQANSEHCRHKIFNASWDIDGQSQEKSLFGMIK
NTYQMHNEGVLSAYKDNAAVIVGNVAGRFYPNPETRQYGAVQEPVHILMKVETHNHPTAI
APFPGASTGSGGEIRDEGATGRGAKPKAGLTGFTVSNLQIPGFEQPWEKPYGKPERIVNA
LDIMIEGPLGGAAFNNEFGRPALTGYFRTFEQSITTPRGEEVRGYHKPIMLAGGMGNIRA
DHVQKGEILVGSKLIVLGGPAMLIGLGGGAASSMATGTSSADLDFASVQRENPEMERRCQ
EVIDRCWQLGEHNPISFIHDVGAGGLSNAFPELVNDGGRGGRFELRNIPNDEPGMAPHEI
WSNESQERYVLAVGPADFERFQAICERERCPFAVVGEATAEPQLTVTDSHFGNSPVDMPL
EVLLGKAPRMHRSAVREAELGDDFDPSTLDLAESIERVLHHPAVASKSFLITIGDRTITG
LVARDQMVGPWQVPVADVAVTATSFDVYTGEAMAMGERTPLALLDAPASGRMAIGETLTN
IAASRIGKISDIKLSANWMSAAGHPGEDARLYDTVKAVGMELCPELGITIPVGKDSMSMA
TRWSDEGVDKSVTSPLSLIVTGFAPVTDIRQTLTPQLRMDKGTTDLILIDLGRGQNRMGA
SILAQVHGKLGSQAPDVDDAEDLKAFFAVIQGLNADGHLLAYHDRSDGGLLTTVVEMAFA
GHCGLSLTLDSVAESSAEIPAILFNEELGAVIQVRQDATPDILAQFSAAGLADCVSVIGQ
PINNAHINITFNGDTVFEGQRSLLQRQWAETSYQIQRLRDNADCAEQEFDALLEEDNPGL
SAKLSYDVNHDVAAPYIKKGIRPQVAVLREQGVNGQVEMAAAFDRAGFNAIDVHMSDILA
GRVDLNDFKGMVACGGFSYGDVLGAGEGWAKSALFNSRARDAFQGFFERTDSFTLGVCNG
CQMMSNLHELIPGSEFWPHFVRNRSEQFEARVAMVQVQESNSIFLQGMAGSRMPIAIAHG
EGHAEFESEEALLEADLSGCVSLRFVDNHGKVTETYPANPNGSPRGITGLTSRDGRVTIM
MPHPERVFRAVQNSWRSDDWNEDAPWMRMFRNARVWVN