Protein Info for Pf6N2E2_5401 in Pseudomonas fluorescens FW300-N2E2

Annotation: phospholipase/carboxylesterase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 218 PF02230: Abhydrolase_2" amino acids 3 to 214 (212 residues), 154 bits, see alignment E=1.1e-48 PF12697: Abhydrolase_6" amino acids 17 to 147 (131 residues), 29.1 bits, see alignment E=3.2e-10 PF01738: DLH" amino acids 89 to 199 (111 residues), 21.1 bits, see alignment E=4.1e-08

Best Hits

Swiss-Prot: 79% identical to EST2_PSEFL: Carboxylesterase 2 (estB) from Pseudomonas fluorescens

KEGG orthology group: K06999, (no description) (inferred from 99% identity to pba:PSEBR_a4578)

Predicted SEED Role

"phospholipase/carboxylesterase family protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A5P7 at UniProt or InterPro

Protein Sequence (218 amino acids)

>Pf6N2E2_5401 phospholipase/carboxylesterase family protein (Pseudomonas fluorescens FW300-N2E2)
MTDPLILEPSSTADACVIWLHGLGADRFDFLPVAEMLQQSLLTTRFVLPQAPTQPVTING
GYEMPSWYDIRALSPARAIDEQQLEASAQRVIDLIETQRASGIDASRIFLAGFSQGGAVV
YHTAFVKWQGPLGGVVALSTYAPTFSDELQLSASQQRIPVLALHGQYDEVVLNPMGRTAK
EYLKQHGVTVTWQEYPMGHEVLPEEIRDIGTWLAERLR