Protein Info for Pf6N2E2_5357 in Pseudomonas fluorescens FW300-N2E2

Annotation: UDP-N-acetylmuramate--alanine ligase (EC 6.3.2.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01225: Mur_ligase
  • amino acids 18 to 116 (99 residues), 107.9 bits, E=4.2e-35
• TIGR01082: UDP-N-acetylmuramate--L-alanine ligase
  • amino acids 18 to 467 (450 residues), 561.5 bits, E=8.4e-173
• PF08245: Mur_ligase_M
  • amino acids 121 to 301 (181 residues), 93.5 bits, E=2.6e-30
• PF02875: Mur_ligase_C
  • amino acids 323 to 458 (136 residues), 59.7 bits, E=8.1e-20

Best Hits

Swiss-Prot: 97% identical to MURC_PSEPF: UDP-N-acetylmuramate--L-alanine ligase (murC) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K01924, UDP-N-acetylmuramate--alanine ligase [EC: 6.3.2.8] (inferred from 99% identity to pba:PSEBR_a4620)

MetaCyc: 62% identical to UDP-N-acetylmuramate--L-alanine ligase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylmuramate--L-alanine ligase. [EC: 6.3.2.8]

Predicted SEED Role

"UDP-N-acetylmuramate--alanine ligase (EC 6.3.2.8)" in subsystem Peptidoglycan Biosynthesis (EC 6.3.2.8)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (12/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• D-Glutamine and D-glutamate metabolism
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.8

Sequence Analysis Tools

See A0A160A4J6 at UniProt or InterPro

Protein Sequence (486 amino acids)

>Pf6N2E2_5357 UDP-N-acetylmuramate--alanine ligase (EC 6.3.2.8) (Pseudomonas fluorescens FW300-N2E2)
MVENRKAMPQPEMRRIRRIHFVGIGGVGMCGIAEVLLNLGYQVSGSDLKASPVTERLESF
GAQIFIGHRAENAANADVLVVSSAVNTSNPEVATALERRIPVVPRAEMLAELMRYRHGIA
VAGTHGKTTTTSLIASVFAAGGLDPTFVIGGRLNAAGTNAQLGTSRYLIAEADESDASFL
HLQPLVAVVTNIDADHMATYDGDFNKLKKTFVEFLHNLPFYGLAVVCLDDPVVREILPLI
KRPTVTYGFGEDVDVRAINVRQQGMQTFFTVLRPDREPLDVSVNMPGNHNVLNALATICI
ATDEGVSDEAIVQGLSGFQGVGRRFQVYGELPVDGGNVMLVDDYGHHPTEVAAVIKAVRG
GWPERRLVMVYQPHRYSRTRDLYDDFVQVLADANVLLLMEVYPAGEEPIPGADSRQLCHS
IRQRGQLDPIYIERGVELAPLVKPLLRAGDILLCQGAGDIGGLAPKLLKSPLFAGAIVAS
SEGKLK