Protein Info for Pf6N2E2_5351 in Pseudomonas fluorescens FW300-N2E2

Annotation: UDP-N-acetylmuramoylalanyl-D-glutamate--2,6- diaminopimelate ligase (EC 6.3.2.13)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 487 TIGR01085: UDP-N-acetylmuramyl-tripeptide synthetase" amino acids 17 to 479 (463 residues), 523.2 bits, see alignment E=3.5e-161 PF01225: Mur_ligase" amino acids 18 to 94 (77 residues), 40.6 bits, see alignment E=4.2e-14 PF08245: Mur_ligase_M" amino acids 106 to 306 (201 residues), 192.6 bits, see alignment E=1.1e-60 PF02875: Mur_ligase_C" amino acids 329 to 455 (127 residues), 118.7 bits, see alignment E=4.7e-38

Best Hits

Swiss-Prot: 83% identical to MURE_PSESM: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K01928, UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase [EC: 6.3.2.13] (inferred from 99% identity to pba:PSEBR_a4626)

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase (EC 6.3.2.13)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.2.13

Use Curated BLAST to search for 6.3.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A1R6 at UniProt or InterPro

Protein Sequence (487 amino acids)

>Pf6N2E2_5351 UDP-N-acetylmuramoylalanyl-D-glutamate--2,6- diaminopimelate ligase (EC 6.3.2.13) (Pseudomonas fluorescens FW300-N2E2)
MSLSLNKIFAHAGHDLLIRELALDSRNVRAGDLFLAVPGARFDGRAHIADALKRGAAAVA
YEVAGATVLPITDVPLIPVKGLAAQLSDIAGRFYGDPSRHLNLVGVTGTNGKTSVTQLVA
QALDLLGQHCGIVGTLGNGFHGALESGLHTTPNPIAVQATLADLKKAGAKAVAMEVSSHG
LDQGRVTALAFDVAVLTNLSRDHLDYHGTMQAYGEAKAKLFAWNDLKCRVINLDDEFGRQ
LAAEKRESRLITYSLEDASAYLYVRQAQFNDEGVRATLVTPQGEHHLRSTLLGRFNLSNV
LAAIGALLGLDYALDEVLKVLPKLEGPAGRMQRLGGGTQPLVVVDYAHTPDALEKVLTAL
RPHAKGRLLCLFGCGGDRDRGKRPLMAEVVERLADGVLVTDDNPRTEDPQRIFDDIRAGF
TAVDNVTFVAGRGQAIAQLIASASADDVIVLAGKGHEDYQEINGERHAFSDLVEADHALT
AWEVAHA