Protein Info for Pf6N2E2_5332 in Pseudomonas fluorescens FW300-N2E2

Annotation: Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 329 PF01965: DJ-1_PfpI" amino acids 49 to 178 (130 residues), 26.9 bits, see alignment E=5.8e-10 PF00165: HTH_AraC" amino acids 232 to 272 (41 residues), 27.5 bits, see alignment 4e-10 amino acids 287 to 323 (37 residues), 34.8 bits, see alignment 2.1e-12 PF12833: HTH_18" amino acids 246 to 324 (79 residues), 76.4 bits, see alignment E=2.6e-25

Best Hits

KEGG orthology group: None (inferred from 99% identity to pba:PSEBR_a4644)

Predicted SEED Role

"Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A1P5 at UniProt or InterPro

Protein Sequence (329 amino acids)

>Pf6N2E2_5332 Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain (Pseudomonas fluorescens FW300-N2E2)
MHASTPLRRVSILAIDRVFASTLMQAKDFFHLASLRYGKQLGHGLTPAFETRLVSPDGKP
VNSFSDVIMPVDGGLDNTDVIILPAFWDDFATLCQRYPQVLPWLREQHARGAVLCGEATG
VFWLAEAGLLDGKEATTYWRFFNAFKERFPQVHLNQDKHLTDADNLFCAGGTTSACDLYI
YLIERFCGANVAQAVARDILYEVQRSYSPGRIGFGGQKLHQDVIILQIQQWLEEHFADKF
RFEDVAREHGMSIRNFMRRFQTATGDKPLHYLQRLRIETAKGLLSGSRKSIKTISYEVGY
DDASFFARLFRQHTDLSPNQYRQQFQQAA