Protein Info for Pf6N2E2_5296 in Pseudomonas fluorescens FW300-N2E2

Annotation: Threonine synthase (EC 4.2.3.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: K01733, threonine synthase [EC: 4.2.3.1] (inferred from 99% identity to pba:PSEBR_a4690)

Predicted SEED Role

"Threonine synthase (EC 4.2.3.1)" in subsystem Threonine and Homoserine Biosynthesis (EC 4.2.3.1)

MetaCyc Pathways

aspartate superpathway (22/25 steps found)
superpathway of L-isoleucine biosynthesis I (13/13 steps found)
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
superpathway of L-threonine biosynthesis (6/6 steps found)
L-threonine biosynthesis (2/2 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.3.1

Use Curated BLAST to search for 4.2.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A384 at UniProt or InterPro

Protein Sequence (417 amino acids)

>Pf6N2E2_5296 Threonine synthase (EC 4.2.3.1) (Pseudomonas fluorescens FW300-N2E2)
MKNSYREYVLQCLVCDRSYQPHEVSYYCPHCKVDGALDALYDYPALKREWSRDSLAHDHA
RGMWRYDRLMPLSSTQFIPPLLVGNTPLYSRPELLGKGARIKVFVKDESRQPTGSLKDRA
SALAVAHALQSGARTVAVASTGNAASALAGMSASLGLHNVIYLPKSAPREKLAQMQGYGA
EIVLVDGQYDEAFEQCLNACQEHGWYNRTTGINSYMSEGKKTVAFEMCEQLNWQVPDLVF
VPVGNGCILGSVYKGFFDLLQLGWIERIPRLIGVQAEHSNFMYRAWRNDRSMQKTERRPP
TSLASSINVALPRDRLKAMRAVTASGGEFICVSDPSIVAAASRLAASTGVFPEAGAASAF
AGLLKYAEIHPDTPQTAVILITGSGLKDTSIFLSDSTKSQPRVLPHHVTAPLAEALI