Protein Info for Pf6N2E2_5200 in Pseudomonas fluorescens FW300-N2E2

Annotation: fosmidomycin resistance protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 405 transmembrane" amino acids 22 to 44 (23 residues), see Phobius details amino acids 56 to 79 (24 residues), see Phobius details amino acids 93 to 125 (33 residues), see Phobius details amino acids 155 to 175 (21 residues), see Phobius details amino acids 181 to 202 (22 residues), see Phobius details amino acids 225 to 245 (21 residues), see Phobius details amino acids 265 to 284 (20 residues), see Phobius details amino acids 294 to 313 (20 residues), see Phobius details amino acids 319 to 340 (22 residues), see Phobius details amino acids 349 to 369 (21 residues), see Phobius details amino acids 381 to 399 (19 residues), see Phobius details PF07690: MFS_1" amino acids 31 to 261 (231 residues), 80.8 bits, see alignment E=4.6e-27 amino acids 234 to 404 (171 residues), 54.7 bits, see alignment E=4.1e-19

Best Hits

Swiss-Prot: 62% identical to FSR_ECOLI: Fosmidomycin resistance protein (fsr) from Escherichia coli (strain K12)

KEGG orthology group: K08223, MFS transporter, FSR family, fosmidomycin resistance protein (inferred from 99% identity to pba:PSEBR_a4772)

MetaCyc: 62% identical to fosmidomycin efflux pump (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-41

Predicted SEED Role

"fosmidomycin resistance protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161H4Q4 at UniProt or InterPro

Protein Sequence (405 amino acids)

>Pf6N2E2_5200 fosmidomycin resistance protein (Pseudomonas fluorescens FW300-N2E2)
MVISNTQAETPLVATTAQSSPLVMRIIGAVALAHLINDLIQAVLPSIYPMLKDSYGLTFT
QVGLITLTFQLTASLLQPWVGYHTDRHPKPWLLPAGTVCTLIGIVMMSMVGSFALILLAA
GLIGIGSSTFHPEASRVARLASGGRYGLAQSTFQVGGNAGSAFGPLLAAAIIIPYGQGNV
AWFGLFALLALFVLYRISRWYANHLNLFKLKQGQAATHGLSKGRVLSALVVLGLLVFSKY
FYMASFTSYFTFYLIEKFDLSVASSQLHLFLFLGAVAAGTFFGGPIGDRIGRKAVIWFSI
LGVAPFTLILPHVDLLWTSILSVVIGFILASAFSAIVVYAQELVPGNVGMIAGVFFGLMF
GFGGIGAALLGHLADIRGIEYVYWLCSFLPLFGVLAIFLPRTRKA