Protein Info for Pf6N2E2_52 in Pseudomonas fluorescens FW300-N2E2

Annotation: Excinuclease ABC subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 671 TIGR00631: excinuclease ABC subunit B" amino acids 3 to 665 (663 residues), 1087.2 bits, see alignment E=0 PF04851: ResIII" amino acids 13 to 86 (74 residues), 38.9 bits, see alignment E=2.6e-13 PF17757: UvrB_inter" amino acids 158 to 248 (91 residues), 113 bits, see alignment E=1.8e-36 PF00271: Helicase_C" amino acids 433 to 543 (111 residues), 70.1 bits, see alignment E=5.6e-23 PF12344: UvrB" amino acids 550 to 591 (42 residues), 68.9 bits, see alignment 8.2e-23 PF02151: UVR" amino acids 633 to 666 (34 residues), 38.8 bits, see alignment (E = 1.6e-13)

Best Hits

Swiss-Prot: 98% identical to UVRB_PSEF5: UvrABC system protein B (uvrB) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K03702, excinuclease ABC subunit B (inferred from 100% identity to pba:PSEBR_a3877)

MetaCyc: 74% identical to UvrABC excision nuclease subunit B (Escherichia coli K-12 substr. MG1655)
3.1.25.-

Predicted SEED Role

"Excinuclease ABC subunit B" in subsystem DNA repair, UvrABC system

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165YHS2 at UniProt or InterPro

Protein Sequence (671 amino acids)

>Pf6N2E2_52 Excinuclease ABC subunit B (Pseudomonas fluorescens FW300-N2E2)
MSEFQLVTRFQPAGDQPEAIRLMVEGIEAGLAHQTLLGVTGSGKTFSIANVIAQIQRPTL
VLAPNKTLAAQLYGEFKAFFPNNAVEYFVSYYDYYQPEAYVPSSDTFIEKDASINDHIEQ
MRLSATKALLERKDAIIVTTVSCIYGLGSPETYLKMVLHVDRGDKLDQRALLRRLADLQY
TRNDMDFARATFRVRGDVIDIYPAESDLEAIRIELFDDEVESLSAFDPLTGEVIRKLPRF
TFYPKSHYVTPRETLLDAIEGIKVELQERLEYLRSNNKLVEAQRLEQRTRFDLEMILELG
YCNGIENYSRYLSGRPAGAPPPTLYDYLPADALLVIDESHVSVPQVGAMYKGDRSRKETL
VEYGFRLPSALDNRPMRFDEWESVSPQTIFVSATPGNYEAEHAGRVVEQVVRPTGLVDPQ
VEVRPALTQVDDLLSEISKRVALEERVLVTTLTKRMAEDLTDYLADHGVRVRYLHSDIDT
VERVEIIRDLRLGTFDVLVGINLLREGLDMPEVSLVAILDADKEGFLRSERSLIQTIGRA
ARNLNGRAILYADRITGSMERAIGETERRRDKQIAFNLANGITPKGVFKDVADIMEGATV
PGSRSKKRKGMAKAAEESARYEAELRSPSEITKRIRQLEEKMYQLARDLEFEAAAQLRDE
IGKLRERLLVV