Protein Info for Pf6N2E2_5188 in Pseudomonas fluorescens FW300-N2E2

Annotation: Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 TIGR01575: ribosomal-protein-alanine acetyltransferase" amino acids 14 to 144 (131 residues), 144.7 bits, see alignment E=9.1e-47 PF00583: Acetyltransf_1" amino acids 18 to 122 (105 residues), 51.8 bits, see alignment E=2.5e-17 PF13673: Acetyltransf_10" amino acids 40 to 128 (89 residues), 48.2 bits, see alignment E=2.6e-16 PF13508: Acetyltransf_7" amino acids 42 to 123 (82 residues), 48.4 bits, see alignment E=2.5e-16 PF08445: FR47" amino acids 64 to 125 (62 residues), 33.1 bits, see alignment E=1.1e-11

Best Hits

Swiss-Prot: 43% identical to RIMI_ECO57: [Ribosomal protein S18]-alanine N-acetyltransferase (rimI) from Escherichia coli O157:H7

KEGG orthology group: K03789, ribosomal-protein-alanine N-acetyltransferase [EC: 2.3.1.128] (inferred from 99% identity to pba:PSEBR_a4781)

MetaCyc: 43% identical to protein N-acetyltransferase RimI (Escherichia coli K-12 substr. MG1655)
2.3.1.128-RXN [EC: 2.3.1.266]; 2.3.1.- [EC: 2.3.1.266]

Predicted SEED Role

"Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-)" in subsystem Bacterial RNA-metabolizing Zn-dependent hydrolases or Conserved gene cluster associated with Met-tRNA formyltransferase or Ribosome biogenesis bacterial (EC 2.3.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.-, 2.3.1.128

Use Curated BLAST to search for 2.3.1.- or 2.3.1.128 or 2.3.1.266

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9X0L1 at UniProt or InterPro

Protein Sequence (150 amino acids)

>Pf6N2E2_5188 Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-) (Pseudomonas fluorescens FW300-N2E2)
MSDAVSFRPMTEADLDAVLKIEYAAYSHPWTRGIFLDGLGKYQIWLMFEGQQQVGHGVVQ
IILDEAHLLNITVKPENQGRGLGLRLLEHLMSIAYKAEARECFLEVRDSNRTAFRLYERY
GFNEVGRRRDYYPAVGGREDAVVMACTLVD