Protein Info for Pf6N2E2_5108 in Pseudomonas fluorescens FW300-N2E2

Annotation: Riboflavin kinase (EC 2.7.1.26) / FMN adenylyltransferase (EC 2.7.7.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00083: riboflavin biosynthesis protein RibF
  • amino acids 2 to 259 (258 residues), 264.4 bits, E=6.8e-83
• PF06574: FAD_syn
  • amino acids 2 to 120 (119 residues), 126.8 bits, E=7.2e-41
• PF01687: Flavokinase
  • amino acids 138 to 259 (122 residues), 141.3 bits, E=1.8e-45

Best Hits

Swiss-Prot: 86% identical to RIBF_PSEFL: Bifunctional riboflavin kinase/FMN adenylyltransferase (ribF) from Pseudomonas fluorescens

KEGG orthology group: K11753, riboflavin kinase / FMN adenylyltransferase [EC: 2.7.1.26 2.7.7.2] (inferred from 95% identity to pba:PSEBR_a4858)

MetaCyc: 54% identical to bifunctional riboflavin kinase / FMN adenylyltransferase (Escherichia coli K-12 substr. MG1655)
FAD synthetase. [EC: 2.7.7.2]; Riboflavin kinase. [EC: 2.7.7.2, 2.7.1.26]

Predicted SEED Role

"Riboflavin kinase (EC 2.7.1.26) / FMN adenylyltransferase (EC 2.7.7.2)" in subsystem Riboflavin, FMN and FAD metabolism (EC 2.7.1.26, EC 2.7.7.2)

MetaCyc Pathways

• adenosylcobalamin biosynthesis II (aerobic) (31/33 steps found)
• flavin biosynthesis I (bacteria and plants) (8/9 steps found)
• flavin biosynthesis III (fungi) (8/9 steps found)
• flavin salvage (2/2 steps found)
• 5,6-dimethylbenzimidazole biosynthesis I (aerobic) (2/3 steps found)
• flavin biosynthesis II (archaea) (6/10 steps found)
• roseoflavin biosynthesis (1/7 steps found)

KEGG Metabolic Maps

• Riboflavin metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.26 or 2.7.7.2

Sequence Analysis Tools

See A0A160A359 at UniProt or InterPro

Protein Sequence (266 amino acids)

>Pf6N2E2_5108 Riboflavin kinase (EC 2.7.1.26) / FMN adenylyltransferase (EC 2.7.7.2) (Pseudomonas fluorescens FW300-N2E2)
MPSCVVIFEPQPREFFAPDTAPARLARLRDKLQLLAAEGVDRVLCLAFNQRLSKLSAAEF
VDTILVDGLGVKHLEVGDDFRFGCDRSGDFDYLQQAGIVQGFTVEAAQTVEIDGLRVSST
QVRNALAAADFELAERLLGRPYRIAGRILHGQKLARQLGTPTANVQLKRRRVPLTGVFLV
SVQIDGKTWPGVANIGVRPTVQGDGKAHLEVHVLDFAGDLYDRRLTVVFHQKLREEQRFA
SLEALKTAIHADIAAARALVATSANR