Protein Info for Pf6N2E2_5083 in Pseudomonas fluorescens FW300-N2E2
Annotation: Serine hydroxymethyltransferase (EC 2.1.2.1)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: None (inferred from 99% identity to pba:PSEBR_a4878)Predicted SEED Role
"Serine hydroxymethyltransferase (EC 2.1.2.1)" in subsystem Folate Biosynthesis or Glycine Biosynthesis or Glycine and Serine Utilization or LMPTP YwlE cluster or Photorespiration (oxidative C2 cycle) or Serine-glyoxylate cycle or Serine Biosynthesis (EC 2.1.2.1)
MetaCyc Pathways
- folate transformations III (E. coli) (9/9 steps found)
- folate transformations II (plants) (10/11 steps found)
- glycine betaine degradation III (7/7 steps found)
- superpathway of L-serine and glycine biosynthesis I (4/4 steps found)
- dTMP de novo biosynthesis (mitochondrial) (3/3 steps found)
- glycine degradation (3/3 steps found)
- folate polyglutamylation (4/5 steps found)
- glycine biosynthesis I (1/1 steps found)
- glycine betaine degradation I (6/8 steps found)
- folate transformations I (9/13 steps found)
- photorespiration II (6/10 steps found)
- purine nucleobases degradation II (anaerobic) (16/24 steps found)
- photorespiration I (5/9 steps found)
- photorespiration III (5/9 steps found)
- glycine betaine degradation II (mammalian) (1/4 steps found)
- formaldehyde assimilation I (serine pathway) (6/13 steps found)
KEGG Metabolic Maps
- Cyanoamino acid metabolism
- Glycine, serine and threonine metabolism
- Methane metabolism
- One carbon pool by folate
Isozymes
Compare fitness of predicted isozymes for: 2.1.2.1
Use Curated BLAST to search for 2.1.2.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A160A2Y3 at UniProt or InterPro
Protein Sequence (1258 amino acids)
>Pf6N2E2_5083 Serine hydroxymethyltransferase (EC 2.1.2.1) (Pseudomonas fluorescens FW300-N2E2) LKSALATLVLLLLGLLFWQLLDQLRETQQQQRQYTIDYTADLAAQVSLNMALNAQIALNL LPIVEQPQTGDEQRALVRKLQQSLPELRSLVLLSPSGRVLSDSDADSHDADYLGDLVRRS RAQAHYFSNAEDGSVVHLLLHQASGSSRGYWALRLSPNFFSTLTKQADMGLRPLWLVENR LNQQIISRDESLPSSSPTHLSPDDLENTVLTVPLSSSDWQLRGLFDRRQVIEQLLPAFIG KCLLGLAFSLLPVIALLNMRRRQRQLHEGRRRYQDIFEGTGVALCVLDLSGLKAFFERAG LHTGDQLRAWTKTPHQLEQLRQEVHVTEVNQMALKLLNVDSCDQAWQLLVGKGTQDNNPV GSKLLEALLDQHKQLELEIKLQDAHGRDQHLWLVLRLPEKQQDHNAVILSISDITSRKLI ELSLLEREGFWSDVVRTVPDHLYVQDVISQRMIFSNHHLGQTLGYDRTELHQMGEYFWEI LLHSDDADHYHSLRQAQRRGGYTQLLQCQLRFRHRNGKWRCFDIREQALARDRDNQVTRI IGVAKDVTEQIEASESLRDSEQRYRMLAESISDVIFSTDNKLSLNYVSPSVQAVLGYTAD WIFQNGWQSIIANPQQLTGIYSLMDRVSKALDKPDQLAELRNQMQTQLFLFDCLRADGRK IPIELRLVLVWDEHGAFEGVLGVGRDISQQRRAEKDLRMAATVFEHSTSAILITDPAGYI VQANEAFSRVSGYAVSQVLDQLPNMLTVDEQQEAHLRYVLKQLQQHSTWEGEVWLKRRNG EHYPAWVGITAVLDDEGDLASYVCFFSDISERKASEQRIHRLAYYDALTHLPNRTLFQDR LHTALQSADRQKTWVVLMFLDLDRFKPINDSLGHAAGDRMLKEMATRLLGCVADDDTVAR MGGDEFTLLLQPRASREMALNRAINVAEQILASLVRPFVLEGREFFVTASIGIALSPQDG NELSQLMKNADTAMYHAKERGKNNFQFYQADMNASALERLELESDLRHALEQNEFVLYYQ PQFSGDGKRLTGAEALLRWRHPRRGLVPPGDFIPVLEELGLVVDVGDWVITEACRQLRTW HQAKVRVPKVSVNISARQFSDGQLGTRIANILRSTGLPPACLELELTESILMREVSEAMQ ILAGLKNLGLSIAVDDFGTGYSSLNYLKQFPIDVLKIDRTFVDGLPSGEQDAQIARAIIA MAHSLNLAVIAEGVETHEQLDFLREHGCDEVQGYLFGRPMPANRFEAQFSNDALFMLD