Protein Info for Pf6N2E2_5026 in Pseudomonas fluorescens FW300-N2E2

Annotation: Acyl-CoA dehydrogenase (EC 1.3.8.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 549 PF18158: AidB_N" amino acids 14 to 168 (155 residues), 204.6 bits, see alignment E=2.3e-64 PF02770: Acyl-CoA_dh_M" amino acids 182 to 279 (98 residues), 51.8 bits, see alignment E=1.9e-17 PF00441: Acyl-CoA_dh_1" amino acids 290 to 444 (155 residues), 92.2 bits, see alignment E=9.3e-30 PF08028: Acyl-CoA_dh_2" amino acids 316 to 426 (111 residues), 33.1 bits, see alignment E=1.6e-11 PF22924: ACOX_C_alpha1" amino acids 372 to 444 (73 residues), 34.9 bits, see alignment E=3.4e-12

Best Hits

KEGG orthology group: K09456, putative acyl-CoA dehydrogenase (inferred from 99% identity to pba:PSEBR_a4937)

Predicted SEED Role

"Acyl-CoA dehydrogenase (EC 1.3.8.7)" (EC 1.3.8.7)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.7

Use Curated BLAST to search for 1.3.8.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A4Q9 at UniProt or InterPro

Protein Sequence (549 amino acids)

>Pf6N2E2_5026 Acyl-CoA dehydrogenase (EC 1.3.8.7) (Pseudomonas fluorescens FW300-N2E2)
MNLHQFAETHDVTNQPPSLDGANLYRIDLPLQQWSQRFGAGWAQARIDAYGALAGGPLME
AGFLANQNKPVFSSHDRYGHRIDLVEFHPAYHQLMRTAVEHGLPSLPWAHPQPGAHVARA
SMTYLHSQAEAGTGCPLTMTFASVPALRLQPDLAEHWVPKVLATEYDPRNVGMAHKAGVT
LGMAMTEKQGGTDVRANTTKAYPVGASGPGQAYELVGHKWFCSAPMCDAFLTLAQTDKGL
TCFLLPRHRPDDTRNQFYIQRLKNKLGNCSNASSEVEFRGALAWMVGEEGRGVPTIIEMV
AMTRFDCMVGSSALMRQALTQASHHCAHRKVGGKLLSEQPLMQNVLADLALESEAALALS
LRMGRALDHLSDEHEAKFARLVTAVGKYWICKRAPAMINEAAECMGGAGYVEDSILPRLY
REAPVNSTWEGSGNVQCLDVLRALSKEPGVLEVLFSELGDGHGDKRLAGHIHQLHAAFKD
TDDIQYRARQLTEDIAVGLQAKLLLEAGNSDVSDAFIASRLGSVGRVYGALPRGLNVEAI
VTRSTPHGF