Protein Info for Pf6N2E2_4958 in Pseudomonas fluorescens FW300-N2E2
Annotation: Cystathionine gamma-synthase (EC 2.5.1.48)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 46% identical to MEGL_FUSNP: L-methionine gamma-lyase (mgl) from Fusobacterium nucleatum subsp. polymorphum
KEGG orthology group: None (inferred from 99% identity to pba:PSEBR_a5002)MetaCyc: 43% identical to MdeA (Pseudomonas putida)
Methionine gamma-lyase. [EC: 4.4.1.11]
Predicted SEED Role
"Cystathionine gamma-synthase (EC 2.5.1.48)" in subsystem Methionine Biosynthesis (EC 2.5.1.48)
MetaCyc Pathways
- superpathway of branched chain amino acid biosynthesis (17/17 steps found)
- aspartate superpathway (22/25 steps found)
- superpathway of L-isoleucine biosynthesis I (13/13 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
- L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
- L-methionine degradation II (3/3 steps found)
- superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)
- L-methionine biosynthesis II (5/6 steps found)
- superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
- superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
- superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
- superpathway of L-threonine metabolism (13/18 steps found)
- L-threonine degradation I (4/6 steps found)
- superpathway of L-cysteine biosynthesis (fungi) (4/6 steps found)
- L-methionine biosynthesis I (3/5 steps found)
- homocysteine and cysteine interconversion (2/4 steps found)
- hypoglycin biosynthesis (4/14 steps found)
- superpathway of seleno-compound metabolism (6/19 steps found)
KEGG Metabolic Maps
- Biosynthesis of plant hormones
- Cysteine metabolism
- Methionine metabolism
- Selenoamino acid metabolism
- Sulfur metabolism
Isozymes
Compare fitness of predicted isozymes for: 2.5.1.48
Use Curated BLAST to search for 2.5.1.48 or 4.4.1.11
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A160A4S9 at UniProt or InterPro
Protein Sequence (417 amino acids)
>Pf6N2E2_4958 Cystathionine gamma-synthase (EC 2.5.1.48) (Pseudomonas fluorescens FW300-N2E2) MNNKPDSTGLDNAGAGTRAVWGGEQVRHPYNATQTPIVASAAYGYDDIDVWYDVALGKAP GFIYSRMSNPTVETLEAKIRELEMAESAVAFSSGMAAISSVLYTFLAHGDRVVSTKDSYG GTNKIFEEFLPRTGVAVTLCETFDHDDLEREIAKGCQVLYLETPTNPTLKILDIPRLVAA AKRVGAVVVADNTFATPLNQSPLALGVDVVIHSATKFLSGHGDVLGGLVCGSEALMAQVR HYREINGASLDPFSAYLIIRGMKTLALRMRQQQHSARALAEFLCTEPLVEAVNYPGLPSH PNHAVACAQMSGFGAIVSFVLVGGMDTVKLLLPRLRFAHCAGNLGAVETIYGPARTTSHV ENTLEERLALGISEGLVRVSVGIEDTDDLLDDLKQAFAFVKKTLTPQLNEAPIETAS