Protein Info for Pf6N2E2_4898 in Pseudomonas fluorescens FW300-N2E2

Annotation: Biotin carboxylase (EC 6.3.4.14)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: None (inferred from 98% identity to pba:PSEBR_a5060)

Predicted SEED Role

"Biotin carboxylase (EC 6.3.4.14)" (EC 6.3.4.14)

MetaCyc Pathways

biotin-carboxyl carrier protein assembly (4/4 steps found)
3-hydroxypropanoate cycle (6/13 steps found)
glyoxylate assimilation (6/13 steps found)
3-hydroxypropanoate/4-hydroxybutanate cycle (9/18 steps found)
superpathway of the 3-hydroxypropanoate cycle (7/18 steps found)

KEGG Metabolic Maps

Fatty acid biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.3.4.14

Use Curated BLAST to search for 6.3.4.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A3T9 at UniProt or InterPro

Protein Sequence (367 amino acids)

>Pf6N2E2_4898 Biotin carboxylase (EC 6.3.4.14) (Pseudomonas fluorescens FW300-N2E2)
MTPVNRNKRVVAYSVNPQAPRHEVETNRALAQWLAQILGWEYGDSHDPSAGHDLYLLPTQ
TLIGTETLMRLGVKGPEDVWGGYVEHDFICTKAITHGLLNKDAVAPKGWSPLFAKLTRGA
VLDGVTVFSLKDARPAAEHLLYTGPIRLKPIHACAGRGQRLIKSLAQFDEIVAGPDAQAL
FRDGVALEQHLNEVKTQSVGQSFINGKVLSYCGVQHLTHDSEGVEVYGGSDLLIVQGGYI
ELLRLELPDDVREAVRLAQVFDDAASQAYPSFFASRRNYDIAQGLDAAGQPRGGVLEQSW
RMGGASSAELAALQVFVEAPQTSAVRVSSVETYTEQPLPPGAREVYRGAAHNGDFLLKYV
TVQSYDG