Protein Info for Pf6N2E2_4799 in Pseudomonas fluorescens FW300-N2E2

Updated annotation (from data): ycgB component of nitrogen-related signalling system (of yeaGH-ycgB)
Rationale: conserved cofitness; yeaG is a protein kinase
Original annotation: FIG004684: SpoVR-like protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 521 PF04293: SpoVR" amino acids 17 to 435 (419 residues), 609.2 bits, see alignment E=3.3e-187 PF24755: YcgB" amino acids 439 to 489 (51 residues), 86.7 bits, see alignment 8.2e-29

Best Hits

Swiss-Prot: 68% identical to YCGB_ECOLI: Uncharacterized protein YcgB (ycgB) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 99% identity to pba:PSEBR_a5144)

Predicted SEED Role

"FIG004684: SpoVR-like protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165ZLZ9 at UniProt or InterPro

Protein Sequence (521 amino acids)

>Pf6N2E2_4799 ycgB component of nitrogen-related signalling system (of yeaGH-ycgB) (Pseudomonas fluorescens FW300-N2E2)
MTAKKEQKRQPISTGSEWTFELIKAYDREISRIADRYALDTYPNQIEVITAEQMMDAYAS
VGMPLGYHHWSYGKHFLSTEKSYSRGQMGLAYEIVINSDPCIAYLMEENTICMQALVVAH
ACYGHNSFFKGNYLFRTWTDASSIIDYLVFAKQYIMQCEERHGIDAVEDLLDSCHALMNY
GVDRYKRPYPISAEEERRRQKDREEHLQKQINDLWRTIPKGADKYNEKDNARFPAEPQEN
ILYFIEKHAPLLEPWQREIVRIVRKIAQYFYPQRQTQVMNEGWATFWHYTLMNDLYDEGL
VTDGFMMEFLTSHTSVVFQPGFDSPYYSGINPYALGFAMYRDIRRMCENPTEEDRRWFPE
IAGSDWLSAIKFAMSSFKDESFILQYLSPKVIRDLKLFSILDDDQKDDLLVPAIHDEGGY
RIIRETLAAQYNLGNREPNVQIYSIDRRGDRSLTLRHQAHNRKPLGDSTDEVLKHLHRLW
GFDIHLETLQGDQVMKTHHVPPRGEQAEGDYGRLDLAVIHL