Protein Info for Pf6N2E2_4768 in Pseudomonas fluorescens FW300-N2E2

Annotation: Acyl-CoA dehydrogenase (EC 1.3.8.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 598 transmembrane" amino acids 520 to 537 (18 residues), see Phobius details PF02771: Acyl-CoA_dh_N" amino acids 40 to 156 (117 residues), 54.3 bits, see alignment E=4.2e-18 PF02770: Acyl-CoA_dh_M" amino acids 162 to 270 (109 residues), 46.9 bits, see alignment E=6.5e-16 PF00441: Acyl-CoA_dh_1" amino acids 281 to 449 (169 residues), 79.1 bits, see alignment E=9.6e-26 PF22924: ACOX_C_alpha1" amino acids 310 to 445 (136 residues), 31.8 bits, see alignment E=3e-11 PF12806: Acyl-CoA_dh_C" amino acids 466 to 592 (127 residues), 137.3 bits, see alignment E=7.9e-44

Best Hits

KEGG orthology group: None (inferred from 96% identity to pba:PSEBR_a5173)

Predicted SEED Role

"Acyl-CoA dehydrogenase (EC 1.3.8.7)" (EC 1.3.8.7)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.7

Use Curated BLAST to search for 1.3.8.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A3I7 at UniProt or InterPro

Protein Sequence (598 amino acids)

>Pf6N2E2_4768 Acyl-CoA dehydrogenase (EC 1.3.8.7) (Pseudomonas fluorescens FW300-N2E2)
MPEYKAPLRDMRFLIDHVFDFHGRYAELGVSDASPDMVSAILEEAARFCENVLAPLNRSG
DEEGCHFDNGVVTTPTGFKQAFAQYVEGGWHGLAADPAYGGLGLPSSLGLVISEMIGSSN
TSWGMYPGLTHGAMSAIHAHGTSEQKQTYLSKLTAGQWTGTMCLTEAHCGTDLGIIKTRA
VPQADGSHAISGSKIFISAGEHDMSDNIIHLVLAKLPDAPAGTKGISLFIVPKFLPDARG
EAGARNGVSCGSIEHKMGIKASATCVLNFDGAKGFLIGEPNKGLNCMFTMMNHARLGTGM
QGLCLGEASFQGAIRYANDRLQMRALTGPKAPEKVADPIIVHPDVRRMLLTMKAFNEGNR
ALTYFTAQLLDTAHLSPDETARQEAEDLLAFLTPICKAFMTDTGLEVTNHGMQVFGGHGF
IREWGMEQLVRDCRIAPIYEGTNGIQALDLLGRKVLGSQGRLLRGFTKIVHKFCAANAQH
PQLKAYVAQLDGLNQQWGELTTRVGMAAMKNPDEVGAASVDYLMYSGYIILAYLWLRMAL
VAQSQLDSGNGDGDFCQAKLATCEFYFKRLLPRTTAHRAAVEAGSDCLMKLPAELFAL