Protein Info for Pf6N2E2_4714 in Pseudomonas fluorescens FW300-N2E2

Annotation: GbcB Glycine betaine demethylase subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 388 PF00970: FAD_binding_6" amino acids 47 to 138 (92 residues), 58.9 bits, see alignment E=8.1e-20 PF00175: NAD_binding_1" amino acids 151 to 256 (106 residues), 49.9 bits, see alignment E=6.9e-17 PF00111: Fer2" amino acids 310 to 381 (72 residues), 50.3 bits, see alignment E=2.8e-17

Best Hits

KEGG orthology group: None (inferred from 99% identity to pba:PSEBR_a5218)

MetaCyc: 84% identical to glycine betaine monooxygenase ferredoxin reductase subunit (Pseudomonas aeruginosa)
RXN-22705 [EC: 1.14.13.251]

Predicted SEED Role

"GbcB Glycine betaine demethylase subunit B" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.14.13.251

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WAR3 at UniProt or InterPro

Protein Sequence (388 amino acids)

>Pf6N2E2_4714 GbcB Glycine betaine demethylase subunit B (Pseudomonas fluorescens FW300-N2E2)
MAVQAPAHNRQHEGRQSAVEISMSNNFLNPVTTQTWANGRHIVRCVKVIQETWDVRTFCF
MADQPIMFFFKPGQFVTLELEIEGQPIMRSYTISSSPSVPYSFSVTIKRVPGGKVSNWLH
DTLHEGQELAVHGPVGLFNAIDFPSPKVLYLSGGVGITPCMSMARWFYDTNANVDMTFIH
SARSPKDIIYHRELEHMASRIDNFSLHLICEKHGLGEPWAGYRGYLNHKMLELMVPDFLE
REVFCCGPTPYMTAVKRLLEAAGYDMKRYHEESFGATPPEARADAVEQAEQAADAPEVDA
ADLHLVEFTSSGKSIRVGPGETVHAAAAKLGMMIPKACGMGICGTCKVLKLGGEVEMDHN
GGITEDDEAEGYILSCCSVPKGDVRIEF